BISC220/S10: Media Recipes

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Wellesley College     BISC 220     Cellular Physiology

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Cytoskeleton Series Media Recipes

Solution Composition
PBS (Phosphate Buffered Saline) 1X 2.68 mM KCl
1.46 mM KH2PO4
136.9 NaCl
8.1 mM Na2HPO4
PBST (PBS with Tween 20) 1X PBS with 0.25% Tween 20
Trypsin Stock = 0.25%
PEM Buffer 1 M Pipes
0.05 M EGTA
0.02 M MgCl2 6H2O
Cell Culture Media 1 Packet DMEM Powder
0.15% NaHCO3
10% Newborn Calf Serum
Trypan Blue 0.1% Trypan Blue in water
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