BISC220/S11:Series 3

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(Schedule of Experiments)
Current revision (10:31, 30 March 2011) (view source)
(Schedule of Experiments)
 
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[[BISC220/S11: Mod 3 Lab 8  | Lab 8: Cell Culture]]<br>
[[BISC220/S11: Mod 3 Lab 8  | Lab 8: Cell Culture]]<br>
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! 8
! 8
| 3/28 - 3/30
| 3/28 - 3/30
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| Induction to Cell Culture
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| Introduction to Cell Culture
| Cell passage and maintenance <br> Work on Paper 2
| Cell passage and maintenance <br> Work on Paper 2
|-
|-
! 9  
! 9  
| 4/5 - 4/7
| 4/5 - 4/7
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| 1) <br> 2) Paper 2 due
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| 1) Isolation of HL-60 genomic DNA<br> 2) Agarose Gel Electrophoresis <br> 3) '''Paper 2 due'''
| Find paper for Presentations
| Find paper for Presentations
|-
|-
!10
!10
| 4/12 - 4/14
| 4/12 - 4/14
-
|  
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| 1) Preparation of Protein Extracts<br> 2) SDS-PAGE Gel <br> 3) Examine fixed HL-60 cells
| Work on Presentations and Final Paper
| Work on Presentations and Final Paper
|-
|-
!11
!11
| 4/20 - 4/21 (4/26 - Tues.)
| 4/20 - 4/21 (4/26 - Tues.)
-
|
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| PARP-1 Western Blot
| Work on Presentations and Final Paper
| Work on Presentations and Final Paper
|-
|-

Current revision

Wellesley College     BISC 220     Cellular Physiology

Home        Lab Calendars/Point Distribution        Assignments        Resources       
Enzymes        Secretory Pathway        Apoptosis        OWW Basics

Lab 8: Cell Culture
Lab 9: Apoptosis - DNA
Lab 10: Apoptosis - Protein 1
Lab 11: Apoptosis - Protein 2
Lab 12: Imaging Presentations
Media Recipes

Schedule of Experiments

Lab # Dates Activity Outside lab time
8 3/28 - 3/30 Introduction to Cell Culture Cell passage and maintenance
Work on Paper 2
9 4/5 - 4/7 1) Isolation of HL-60 genomic DNA
2) Agarose Gel Electrophoresis
3) Paper 2 due
Find paper for Presentations
10 4/12 - 4/14 1) Preparation of Protein Extracts
2) SDS-PAGE Gel
3) Examine fixed HL-60 cells
Work on Presentations and Final Paper
11 4/20 - 4/21 (4/26 - Tues.) PARP-1 Western Blot Work on Presentations and Final Paper
12 4/27 - 4/28 (5/3 - Tues.) Imaging Presentations Final Paper due for everyone - Friday, May 6, 2011
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