BISC220/S11: Mod 1 Media: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 27: | Line 27: | ||
|- | |- | ||
! Laemmli Solution (2x Sample Buffer) | ! Laemmli Solution (2x Sample Buffer) | ||
| 0.125 M Tris | | 0.125 M Tris, pH 6.7 <br> 4% SDS <br> 20% glycerol <br> 10% β-mercaptoethanol | ||
|- | |- | ||
! Gel Running Buffer | ! Gel Running Buffer | ||
Latest revision as of 09:48, 16 February 2011
β-galactosidase Series Media Recipes
| Solution | Composition |
|---|---|
| Luria Broth | 1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar (only in plates) |
| Ampicillin | Stock = 25 mg/ml in water |
| IPTG (Isopropyl β-D-1-thiogalactopyranoside) | Stock 1 mg/ml |
| Na2CO3 | Stock 1M |
| Z-buffer | 60 mM Na2HPO4 60 mM NaH2PO4 1 mM MgSO4 10 mM HCl 0.27% β-mercaptoethanol |
| ONPG (ortho-Nitrophenyl-β-galactoside) | Stock 4 mg/ml |
| Laemmli Solution (2x Sample Buffer) | 0.125 M Tris, pH 6.7 4% SDS 20% glycerol 10% β-mercaptoethanol |
| Gel Running Buffer | 0.92 M Glycine 0.05 M Tris Base 0.1% SDS |
| GelCode Blue Stain Reagent | Thermo Scientific |
