BISC220/S12:Series 1
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Revision as of 14:01, 3 January 2012
Lab 1: Induction of β-galactosidase and Molecular Modeling
Lab 2: Affinity Chromatography and Specific Activity Determination
Lab 3: Enzyme Kinetics
The Michaelis-Menten Model
Lab 4: Polyacrylamide Gel Electrophoresis
Media Recipes
Lab 5: Science Writing and Figure Design
Schedule of Experiments
| Lab # | Dates | Activity | Outside lab time |
|---|---|---|---|
| 1 | 1/31 - 2/3 | 1) Induction of β-galactosidase 2) Molecular Modeling and Sequence Analysis | Figure creation and brief analysis of the β-galactosidase molecule |
| 2 | 2/7 - 2/10 | 1) Affinity Chromatography 2) Determination of the Specific Activity of β-galactosidase | Calculations based on the results of today's lab |
| 3 | 2/14- 2/17 | Enzyme Kinetics | Calculations based on the results of today's lab |
| 4 | 2/21 - 2/24 | Polyacrylamide gel electrophoresis | 1) Calculations based on the results of today's lab 2) Write β-galactosidase paper |
