BISC220/S12:Series 1
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| - | [[BISC220/ | + | [[BISC220/S12: Mod 1 Lab 1 | Lab 1: Induction of β-galactosidase and Molecular Modeling]]<br> |
| - | [[BISC220/ | + | [[BISC220/S12: Mod 1 Lab 2 | Lab 2: Affinity Chromatography and Specific Activity Determination]]<br> |
| - | [[BISC220/ | + | [[BISC220/S12: Mod 1 Lab 3 | Lab 3: Enzyme Kinetics]]<br> |
| - | [[BISC220/ | + | [[BISC220/S12: The Michaelis-Menten Model | The Michaelis-Menten Model]]<br> |
| - | [[BISC220/ | + | [[BISC220/S12: Mod 1 Lab 4 | Lab 4: Polyacrylamide Gel Electrophoresis]]<br> |
| - | [[BISC220/ | + | [[BISC220/S12: Mod 1 Media | Media Recipes]]<br> |
| - | [[BISC220/ | + | [[BISC220/S12: Mod 1 Science Writing | Science Writing and Figure Design]]<br> |
=='''Schedule of Experiments'''== | =='''Schedule of Experiments'''== | ||
| Line 16: | Line 16: | ||
|- | |- | ||
! 1 | ! 1 | ||
| - | | 1/ | + | | 1/31 - 2/3 |
| 1) Induction of β-galactosidase <br> 2) Molecular Modeling and Sequence Analysis | | 1) Induction of β-galactosidase <br> 2) Molecular Modeling and Sequence Analysis | ||
| Figure creation and brief analysis of the β-galactosidase molecule | | Figure creation and brief analysis of the β-galactosidase molecule | ||
|- | |- | ||
!2 | !2 | ||
| - | | 2/ | + | | 2/7 - 2/10 |
| 1) Affinity Chromatography <br> 2) Determination of the Specific Activity of β-galactosidase | | 1) Affinity Chromatography <br> 2) Determination of the Specific Activity of β-galactosidase | ||
| Calculations based on the results of today's lab | | Calculations based on the results of today's lab | ||
|- | |- | ||
!3 | !3 | ||
| - | | 2/ | + | | 2/14- 2/17 |
| Enzyme Kinetics | | Enzyme Kinetics | ||
| Calculations based on the results of today's lab | | Calculations based on the results of today's lab | ||
|- | |- | ||
!4 | !4 | ||
| - | | 2/ | + | | 2/21 - 2/24 |
| Polyacrylamide gel electrophoresis | | Polyacrylamide gel electrophoresis | ||
| 1) Calculations based on the results of today's lab<br> | | 1) Calculations based on the results of today's lab<br> | ||
2) Write β-galactosidase paper | 2) Write β-galactosidase paper | ||
| - | |||
|} | |} | ||
Current revision
Lab 1: Induction of β-galactosidase and Molecular Modeling
Lab 2: Affinity Chromatography and Specific Activity Determination
Lab 3: Enzyme Kinetics
The Michaelis-Menten Model
Lab 4: Polyacrylamide Gel Electrophoresis
Media Recipes
Science Writing and Figure Design
Schedule of Experiments
| Lab # | Dates | Activity | Outside lab time |
|---|---|---|---|
| 1 | 1/31 - 2/3 | 1) Induction of β-galactosidase 2) Molecular Modeling and Sequence Analysis | Figure creation and brief analysis of the β-galactosidase molecule |
| 2 | 2/7 - 2/10 | 1) Affinity Chromatography 2) Determination of the Specific Activity of β-galactosidase | Calculations based on the results of today's lab |
| 3 | 2/14- 2/17 | Enzyme Kinetics | Calculations based on the results of today's lab |
| 4 | 2/21 - 2/24 | Polyacrylamide gel electrophoresis | 1) Calculations based on the results of today's lab 2) Write β-galactosidase paper |
