BISC220/S12:Series 1

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! 1
! 1
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| 1/26 - 2/1
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| 1/31 - 2/3
| 1) Induction of β-galactosidase <br> 2) Molecular Modeling and Sequence Analysis
| 1) Induction of β-galactosidase <br> 2) Molecular Modeling and Sequence Analysis
| Figure creation and brief analysis of the β-galactosidase molecule
| Figure creation and brief analysis of the β-galactosidase molecule
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|-
!2  
!2  
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| 2/2 - 2/8
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| 2/7 - 2/10
| 1) Affinity Chromatography <br> 2) Determination of the Specific Activity of β-galactosidase
| 1) Affinity Chromatography <br> 2) Determination of the Specific Activity of β-galactosidase
| Calculations based on the results of today's lab
| Calculations based on the results of today's lab
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|-
!3
!3
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| 2/9- 2/15
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| 2/14- 2/17
| Enzyme Kinetics
| Enzyme Kinetics
| Calculations based on the results of today's lab
| Calculations based on the results of today's lab
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|-
!4
!4
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| 2/16 - 2/22
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| 2/21 - 2/24
| Polyacrylamide gel electrophoresis
| Polyacrylamide gel electrophoresis
| 1) Calculations based on the results of today's lab<br>
| 1) Calculations based on the results of today's lab<br>
2) Write β-galactosidase paper
2) Write β-galactosidase paper
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Current revision

Wellesley College     BISC 220     Cellular Physiology

Home        Lab Calendars/Point Distribution        Assignments        Resources       
Enzymes        Secretory Pathway        Apoptosis        OWW Basics

Lab 1: Induction of β-galactosidase and Molecular Modeling
Lab 2: Affinity Chromatography and Specific Activity Determination
Lab 3: Enzyme Kinetics
The Michaelis-Menten Model
Lab 4: Polyacrylamide Gel Electrophoresis
Media Recipes
Science Writing and Figure Design

Schedule of Experiments

Lab # Dates Activity Outside lab time
1 1/31 - 2/3 1) Induction of β-galactosidase
2) Molecular Modeling and Sequence Analysis
Figure creation and brief analysis of the β-galactosidase molecule
2 2/7 - 2/10 1) Affinity Chromatography
2) Determination of the Specific Activity of β-galactosidase
Calculations based on the results of today's lab
3 2/14- 2/17 Enzyme Kinetics Calculations based on the results of today's lab
4 2/21 - 2/24 Polyacrylamide gel electrophoresis 1) Calculations based on the results of today's lab

2) Write β-galactosidase paper

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