BISC220/S12:Series 3: Difference between revisions

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! 8
! 8
| 3/27 - 3/29
| 3/27 - 3/30
| Introduction to Cell Culture
| Introduction to Cell Culture
| Cell passage and maintenance <br> Work on Paper 2
| Cell passage and maintenance <br> Work on Paper 2
|-
|-
! 9  
! 9  
| 4/3 - 4/5
| 4/3 - 4/6
| 1) Isolation of HL-60 genomic DNA<br> 2) Agarose Gel Electrophoresis <br> 3) '''Paper 2 due'''
| 1) Isolation of HL-60 genomic DNA<br> 2) Agarose Gel Electrophoresis <br> 3) '''Paper 2 due'''
| Find paper for Presentations
| Find paper for Presentations
|-
|-
!10
!10
| 4/10 - 4/12
| 4/10 - 4/13
| 1) Preparation of Protein Extracts<br> 2) SDS-PAGE Gel <br> 3) Examine fixed HL-60 cells
| 1) Preparation of Protein Extracts<br> 2) SDS-PAGE Gel <br> 3) Examine fixed HL-60 cells
| Work on Presentations and Final Paper
| Work on Presentations and Final Paper
|-
|-
!11
!11
| 4/17 - 4/19
| 4/17 - 4/27
| PARP-1 Western Blot
| PARP-1 Western Blot
| Work on Presentations and Final Paper
| Work on Presentations and Final Paper
|-
|-
!12
!12
| 4/24 - 4/26 (5/2 - Wed.)
| 5/1 - 5/4
| Imaging Presentations
| Imaging Presentations
| Final Paper due for everyone - Thurs., May 3, 2012
| Final Paper due for everyone - Friday, May 4, 2012
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|-
|}
|}

Latest revision as of 09:33, 3 January 2012

Wellesley College     BISC 220     Cellular Physiology

Home        Lab Calendars/Point Distribution        Assignments        Resources       
Enzymes        Secretory Pathway        Apoptosis        OWW Basics

Lab 8: Cell Culture
Lab 9: Apoptosis - DNA
Lab 10: Apoptosis - Protein 1
Lab 11: Apoptosis - Protein 2
Lab 12: Imaging Presentations
Media Recipes

Schedule of Experiments

Lab # Dates Activity Outside lab time
8 3/27 - 3/30 Introduction to Cell Culture Cell passage and maintenance
Work on Paper 2
9 4/3 - 4/6 1) Isolation of HL-60 genomic DNA
2) Agarose Gel Electrophoresis
3) Paper 2 due
Find paper for Presentations
10 4/10 - 4/13 1) Preparation of Protein Extracts
2) SDS-PAGE Gel
3) Examine fixed HL-60 cells
Work on Presentations and Final Paper
11 4/17 - 4/27 PARP-1 Western Blot Work on Presentations and Final Paper
12 5/1 - 5/4 Imaging Presentations Final Paper due for everyone - Friday, May 4, 2012