BISC220/S12:Series 3

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Wellesley College     BISC 220     Cellular Physiology

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Enzymes        Secretory Pathway        Apoptosis        OWW Basics

Lab 8: Cell Culture
Lab 9: Apoptosis - DNA
Lab 10: Apoptosis - Protein 1
Lab 11: Apoptosis - Protein 2
Lab 12: Imaging Presentations
Media Recipes

Schedule of Experiments

Lab # Dates Activity Outside lab time
8 3/27 - 3/30 Introduction to Cell Culture Cell passage and maintenance
Work on Paper 2
9 4/3 - 4/6 1) Isolation of HL-60 genomic DNA
2) Agarose Gel Electrophoresis
3) Paper 2 due
Find paper for Presentations
10 4/10 - 4/13 1) Preparation of Protein Extracts
2) SDS-PAGE Gel
3) Examine fixed HL-60 cells
Work on Presentations and Final Paper
11 4/17 - 4/27 PARP-1 Western Blot Work on Presentations and Final Paper
12 5/1 - 5/4 Imaging Presentations Final Paper due for everyone - Friday, May 4, 2012
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