BISC220/S13:Series 1: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(New page: {{Template:BISC220/S13}} <div style="padding: 10px; width: 720px; border: 5px solid #E05D1B;"> Lab 1: Induction of β-galactosidase and Molecular Modeling<...)
 
 
Line 16: Line 16:
|-
|-
! 1
! 1
| 1/31 - 2/3
| 1/29 - 2/1
| 1) Induction of β-galactosidase <br> 2) Molecular Modeling and Sequence Analysis
| 1) Induction of β-galactosidase <br> 2) Molecular Modeling and Sequence Analysis
| Figure creation and brief analysis of the β-galactosidase molecule
| Figure creation and brief analysis of the β-galactosidase molecule
|-
|-
!2  
!2  
| 2/7 - 2/10
| 2/4 - 2/8
| 1) Affinity Chromatography <br> 2) Determination of the Specific Activity of β-galactosidase
| 1) Affinity Chromatography <br> 2) Determination of the Specific Activity of β-galactosidase
| Calculations based on the results of today's lab
| Calculations based on the results of today's lab
|-
|-
!3
!3
| 2/14- 2/17
| 2/11- 2/15
| Enzyme Kinetics
| Enzyme Kinetics
| Calculations based on the results of today's lab
| Calculations based on the results of today's lab
|-
|-
!4
!4
| 2/21 - 2/24
| 2/25 - 3/1
| Polyacrylamide gel electrophoresis
| Polyacrylamide gel electrophoresis
| 1) Calculations based on the results of today's lab<br>
| 1) Calculations based on the results of today's lab<br>
2) Write β-galactosidase paper
2) Write β-galactosidase paper
|}
|}

Latest revision as of 06:15, 4 May 2012

Wellesley College     BISC 220     Cellular Physiology

Home        Lab Calendars/Point Distribution        Assignments        Resources       
Enzymes        Secretory Pathway        Apoptosis        OWW Basics

Lab 1: Induction of β-galactosidase and Molecular Modeling
Lab 2: Affinity Chromatography and Specific Activity Determination
Lab 3: Enzyme Kinetics
The Michaelis-Menten Model
Lab 4: Polyacrylamide Gel Electrophoresis
Media Recipes
Science Writing and Figure Design

Schedule of Experiments

Lab # Dates Activity Outside lab time
1 1/29 - 2/1 1) Induction of β-galactosidase
2) Molecular Modeling and Sequence Analysis
Figure creation and brief analysis of the β-galactosidase molecule
2 2/4 - 2/8 1) Affinity Chromatography
2) Determination of the Specific Activity of β-galactosidase
Calculations based on the results of today's lab
3 2/11- 2/15 Enzyme Kinetics Calculations based on the results of today's lab
4 2/25 - 3/1 Polyacrylamide gel electrophoresis 1) Calculations based on the results of today's lab

2) Write β-galactosidase paper