BISC220/S13: Mod 2 Media Recipes: Difference between revisions
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| 25mM Trisma Base <br> 192mM Glycine <br> 20% v/v Methanol | | 25mM Trisma Base <br> 192mM Glycine <br> 20% v/v Methanol | ||
|- | |- | ||
! | ! Immun-Star™ WesternC™ Chemiluminescence Kit | ||
| www. | | http://www.bio-rad.com <br> Catalog #170-5070 | ||
|- | |- | ||
! Strain Source | ! Strain Source |
Latest revision as of 11:10, 8 April 2013
Background Information
Lab 6: Phenotypes of Secretory Mutants
Lab 7: Analyzing Secretion Defects by Western Blotting
Lab 8: Probing and Detecting the Western Blot
Media Recipes
Yeast Series Media Recipes
Solution | Composition |
---|---|
PBS (Phosphate Buffered Saline) 1X | 2.68 mM KCl 1.46 mM KH2PO4 136.9 NaCl 8.1 mM Na2HPO4 |
PBST (PBS with Tween 20) | 1X PBS with 0.25% Tween 20 |
YPD (Yeast Peptone Dextrose | 1% Yeast Extract 2% Peptone 2% Glucose 2% Agar (only in solid media) |
SD (Sabouraud's Dextrose) -Ura | 0.67% Yeast Nitrogen Base 2% Glucose 0.08% -Ura amino acid drop out mix 2% Agar (only in solid media) |
SD-Ura-His | 0.67% Yeast Nitrogen Base 2% Glucose 0.078% -Ura -His amino acid drop out mix 0.003M Histidinol 2% Agar (only in solid media) |
Yeast 5X Protein Sample Buffer | 10% SDS 25% Glycerol 250mM Tris (pH = 6.8) 500mM DTT 0.5% BPB |
Acrylamide Gel Tank Buffer (Gel Running) | 0.92 M Glycine 0.05M Trisma base 0.1% SDS |
Western Blotting Buffer (Nitrocellulose Transfer) | 25mM Trisma Base 192mM Glycine 20% v/v Methanol |
Immun-Star™ WesternC™ Chemiluminescence Kit | http://www.bio-rad.com Catalog #170-5070 |
Strain Source | Elizabeth Valen at Swarthmore College is the original source of the 3 plasmids and 3 strains Jennifer Hood-DeGrenier at Worchester State is the source of the Mat a strain |