BISC220/S13: Mod 2 Media Recipes

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Current revision (14:10, 8 April 2013) (view source)
(Yeast Series Media Recipes)
 
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| 25mM Trisma Base <br> 192mM Glycine <br> 20% v/v Methanol
| 25mM Trisma Base <br> 192mM Glycine <br> 20% v/v Methanol
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|-
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! Amersham ECL Western Blotting Analysis System
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! Immun-Star™ WesternC™ Chemiluminescence Kit
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| www.gelifesciences.com <br> catalog #RPN2109
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| http://www.bio-rad.com <br> Catalog #170-5070
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|-
! Strain Source
! Strain Source

Current revision

Wellesley College     BISC 220     Cellular Physiology

Home        Lab Calendars/Point Distribution        Assignments        Resources       
Enzymes        Secretory Pathway        Apoptosis        OWW Basics

Background Information
Lab 6: Phenotypes of Secretory Mutants
Lab 7: Analyzing Secretion Defects by Western Blotting
Lab 8: Probing and Detecting the Western Blot
Media Recipes

Yeast Series Media Recipes

Solution Composition
PBS (Phosphate Buffered Saline) 1X 2.68 mM KCl
1.46 mM KH2PO4
136.9 NaCl
8.1 mM Na2HPO4
PBST (PBS with Tween 20) 1X PBS with 0.25% Tween 20
YPD (Yeast Peptone Dextrose 1% Yeast Extract
2% Peptone
2% Glucose
2% Agar (only in solid media)
SD (Sabouraud's Dextrose) -Ura 0.67% Yeast Nitrogen Base
2% Glucose
0.08% -Ura amino acid drop out mix
2% Agar (only in solid media)
SD-Ura-His 0.67% Yeast Nitrogen Base
2% Glucose
0.078% -Ura -His amino acid drop out mix
0.003M Histidinol
2% Agar (only in solid media)
Yeast 5X Protein Sample Buffer 10% SDS
25% Glycerol
250mM Tris (pH = 6.8)
500mM DTT
0.5% BPB
Acrylamide Gel Tank Buffer (Gel Running) 0.92 M Glycine
0.05M Trisma base
0.1% SDS
Western Blotting Buffer (Nitrocellulose Transfer) 25mM Trisma Base
192mM Glycine
20% v/v Methanol
Immun-Star™ WesternC™ Chemiluminescence Kit http://www.bio-rad.com
Catalog #170-5070
Strain Source Elizabeth Valen at Swarthmore College is the original source of the 3 plasmids and 3 strains
Jennifer Hood-DeGrenier at Worchester State is the source of the Mat a strain
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