BISC220/S14:Series 1
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Lab 1: Introduction to BISC220 Lab
Lab 2: Induction of β-galactosidase and Molecular Modeling
Lab 3: Affinity Chromatography and Polyacrylamide Gel Electrophoresis
Lab 4: Specific Activity Determination
The Michaelis-Menten Model
Lab 5: Enzyme Kinetics
Media Recipes
Science Writing and Figure Design
Schedule of Experiments
| Lab # | Dates | Activity | Outside lab time |
|---|---|---|---|
| 1 | 1/27 - 1/31 | Introduction to BISC220 Lab | |
| 2 | 2/3 - 2/7 | 1) Induction of β-galactosidase 2) Molecular Modeling and Sequence Analysis |
Figure creation and brief analysis of the β-galactosidase molecule |
| 3 | 2/10 - 2/14 | 1) Affinity Chromatography 2) Polyacrylamide Gel Electrophoresis |
Figure Design and Legend Writing |
| 4 | 2/18- 2/24 | Determination of the Specific Activity of β-galactosidase | Calculations based on the results of today's lab |
| 5 | 2/25 - 3/3 | Enzyme Kinetics | 1) Calculations based on the results of today's lab 2) Prepare for writing workshop |
| 6 | 3/4 - 3/10 | Writing Workshop | Write Final Paper for Enzyme Kinetics |
