Irene Newton at 15:23, 11 September 2010

Irene Newton — Sat, 11 Sep 2010 15:23:51 GMT

←Older revision		Revision as of 15:23, 11 September 2010
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	<b>LAB #1: Learning Sterile Technique and Field Trip to the Cheese Shop</b><br>		<b>LAB #1: Learning Sterile Technique and Field Trip to the Cheese Shop</b><br>

-	Today we will be taking a trip in to Cambridge to visit [http://www.formaggiokitchen.com/ ~~Formagio~~ Kitchen], a famous cheese shop in the area - they even have a cheese cave! Each of you will pick a cheese to be your microbial habitat for the next few weeks. We will sample a large variety and learn about where these cheeses came from. Let's be sure we have representative cheeses from these four main classes: <br>	+	Today we will be taking a trip in to Cambridge to visit [http://www.formaggiokitchen.com/ Formaggio Kitchen], a famous cheese shop in the area - they even have a cheese cave! Each of you will pick a cheese to be your microbial habitat for the next few weeks. We will sample a large variety and learn about where these cheeses came from. Let's be sure we have representative cheeses from these four main classes: <br>

	1. A blue cheese <br>		1. A blue cheese <br>
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	4. A soft, brie-like cheese <br>		4. A soft, brie-like cheese <br>

-	Back in the lab, we'll review sterile technique and inoculate media of various kinds from our cheese rinds. We will isolate a beautiful array of different microbes (both eukaryotic and bacterial) from these cheeses and in the next few weeks you'll be using them to investigate two major microbial community functions: growth interactions and chemical signaling. <br>	+	Back in the lab, we'll review sterile technique and inoculate media of various kinds from our cheese rinds. We will isolate a beautiful array of different microbes (both eukaryotic and bacterial) from these cheeses and in the next few weeks you'll be using them to investigate two major microbial community functions: growth interactions and chemical signaling. If you haven't taken BISC209, or if you feel rusty on your microbiology, you might want to peruse the following link: [http://openwetware.org/wiki/BISC209:Protcols BISC209:Protocols]<br>

-	<br>	+	'''Streaking out our cheese isolates''' <br>
-	</~~div~~>	+	1. Make sure to wear gloves and use proper sterile technique throughout <br>
		+	2. Get 4 NB agar plates and label them with your name, the date, your cheese, and what part of the cheese you are inoculating from <br>
		+	3. Flame your loop to sterilize it <br>
		+	4. Pass your flamed loop over the surface of the cheese - you don't need a chunk! Microbes are invisible - don't get a big scoop of the cheese on your plate as this will lead to over-growth <br>
		+	5. Use your loop to smear out this inoculum over about 1/4 of the plate. Use the image below to help guide you. <br>
		+	6. Reflame the loop and use it to dilute this smear further. Pass the loop through the initial, most dense streak once. Then make a second smear on the plate using the loop. <br>
		+	7. Continue till you've completed the pattern below on all 4 plates <br>
		+	[[Image:Streaking_diagram.jgp]] <br>
		+	8. Place your NB plates agar side up on your bench. We will come back Friday to check on them <br>

Irene Newton at 17:20, 6 August 2010

Irene Newton — Fri, 06 Aug 2010 17:20:57 GMT

←Older revision		Revision as of 17:20, 6 August 2010
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	[[BISC314:People \| <font face="trebuchet ms" style="color:#FFFF00"> '''People''' </font>]]		[[BISC314:People \| <font face="trebuchet ms" style="color:#FFFF00"> '''People''' </font>]]

-	[[BISC314:~~Materials~~ \| <font face="trebuchet ms" style="color:#FFFF00"> '''~~Laboratory Protocols~~''' </font>]]	+	[[BISC314:Full Protocol \| <font face="trebuchet ms" style="color:#FFFF00"> '''Full Protocol''' </font>]]

-	[[BISC314:Schedule \| <font face="trebuchet ms" style="color:#FFFF00"> '''Laboratory Schedule''' </font>]]	+	[[BISC314:Laboratory Schedule \| <font face="trebuchet ms" style="color:#FFFF00"> '''Laboratory Schedule''' </font>]]

-	[[BISC314:~~Help~~ \| <font face="trebuchet ms" style="color:#FFFF00"> '''Lecture Syllabus''' </font>]]	+	[[BISC314:Lecture Syllabus \| <font face="trebuchet ms" style="color:#FFFF00"> '''Lecture Syllabus''' </font>]]

	<!-- Feel free to add or remove items from the navigation bar to customize the header for your class. -->		<!-- Feel free to add or remove items from the navigation bar to customize the header for your class. -->

←Older revision		Revision as of 17:20, 6 August 2010
Line 11:		Line 11:
	[[BISC314:People \| <font face="trebuchet ms" style="color:#FFFF00"> '''People''' </font>]]		[[BISC314:People \| <font face="trebuchet ms" style="color:#FFFF00"> '''People''' </font>]]

-	[[BISC314:~~Materials~~ \| <font face="trebuchet ms" style="color:#FFFF00"> '''~~Laboratory Protocols~~''' </font>]]	+	[[BISC314:Full Protocol \| <font face="trebuchet ms" style="color:#FFFF00"> '''Full Protocol''' </font>]]

-	[[BISC314:Schedule \| <font face="trebuchet ms" style="color:#FFFF00"> '''Laboratory Schedule''' </font>]]	+	[[BISC314:Laboratory Schedule \| <font face="trebuchet ms" style="color:#FFFF00"> '''Laboratory Schedule''' </font>]]

-	[[BISC314:~~Help~~ \| <font face="trebuchet ms" style="color:#FFFF00"> '''Lecture Syllabus''' </font>]]	+	[[BISC314:Lecture Syllabus \| <font face="trebuchet ms" style="color:#FFFF00"> '''Lecture Syllabus''' </font>]]

	<!-- Feel free to add or remove items from the navigation bar to customize the header for your class. -->		<!-- Feel free to add or remove items from the navigation bar to customize the header for your class. -->

BISC314:Lab1 - Revision history

Irene Newton at 15:23, 11 September 2010

Irene Newton at 17:20, 6 August 2010

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