BISC 111/113:Calendar and Assignments
BISC111/113 Fall 2011 Lab Calendar
Monday | Tuesday | Wednesday | Thursday | Friday | |
---|---|---|---|---|---|
August 29 |
August 30 Lab 1 |
August 31 Lab 1 |
September 1 Lab 1 |
September 2 Lab 1 |
|
September 5 Labor Day No Classes |
September 6 |
September 7 Monday Schedule Lab 1 |
September 8 Lab 2 |
September 9 Lab 2 |
|
September 12 Lab 2 |
September 13 Lab 2 |
September 14 Lab 2 |
September 15 Lab 3 |
September 16 Lab 3 |
|
September 19 Lab 3 |
September 20 Lab 3 |
September 21 Lab 3 |
September 22 Lab 4 |
September 23 Lab 4 |
|
September 26 Lab 4 |
September 27 Lab 4 |
September 28 Lab 4 |
September29 Lab 5 |
September 30 Lab 5 |
|
October 3 Lab 5 |
October 4 Lab 5 |
October 5 Lab 6 |
October 6 |
October 7 |
|
October 10 Fall Break No Classes |
October 11 Fall Break No Classes |
October 12 Lab 6 |
October 13 Lab 6 |
October 14 Lab 6 |
|
October 17 Lab 6 |
October 18 Lab 6 |
October 19 Lab 7 |
October 20 Lab 7 |
October 21 Lab 7 |
|
October 24 Lab 7 |
October 25 Lab 7 |
October 26 Lab 8 |
October 27 Lab 8 |
October 28 Lab 8 |
|
October 31 |
November 1 Tanner No Classes |
November 2 Lab 8 |
November 3 Lab 8 |
November 4 Lab 8 |
|
November 7 4 Lab 8 |
November 8 Lab 8 |
November 9 Lab 9 |
November 10 Lab 9 |
November 11 Lab 9 |
|
November 14 Lab 9 |
November 15 Lab 9 |
November 16 Lab 11 |
November 17 Lab 11 |
November 18 Lab 11 |
|
November 21 Lab Practical Lab 10 for all |
November 22 |
November 23 Thanksgiving Break |
November 24 Thanksgiving Break |
November 25 Thanksgiving Break |
|
November 28 Lab 11 |
November 29 Lab 11 |
November 30 |
December 1 Lab 12 |
December 12 Lab 12 |
|
December 5 Lab 12 |
December 6 Lab 12 |
December 7 Lab 12 |
December 8 |
December 9 |
Schedule of Experiments
Section | Lab # | Activities |
---|---|---|
1 | 1-5 | Boot Camp: Metric Measurement; Using Basic Lab Equipment; Making Solutions and Dilutions; Microscopy; Scientific Investigation; Designing & Executing Experiments; Data Analysis & Display; Statistical parameters; Scientific Writing |
2 | 6-7 | Genetics: DNA extraction, Visualization by agarose gel electrophoresis; Genotype/phenotype via Taster SNP data collection and analysis |
3 | 8-10 | Photosynthesis: Extraction of photosynthetic pigments; Spectrophotometry: Beer-Lambert Law, Linear regression analysis; Hill Reaction: Self-designed investigation of factors affecting photosystems |
4 | 11 | Gene Regulation/Enzymology: Invertase enzyme assay |
Lab Practical | 12 | Hands-on skills assessment: |
BISC110/112 Assignments/ Weekly Lab Planner
Series | Lab # | LAB DATES | LAB DESCRIPTION | Assignment/Notes | ||
1 | 1 | Jan. 24 to Jan. 28 |
Practice metric conversions; Practice pipetting; Making Solutions; Microscopy; Examination of Tetrahymena pyriformis |
Homework: Familiarize yourself with the wiki, particularly the information in the BISC110/S11:Resources section of the Lab wiki; Lab 1 Practice Problems downloadable here Media:Lab_1_Practice_Problems110.doc; Read about the process of endocytosis in your textbook; familiarize yourself with phagocytosis in Tetrahymena from the following reference articles: | ||
1 | 2 | Jan. 31 to Feb. 4 |
Tetrahymena Behavior: Phagocytosis; Observations; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Effective Figure Design and Legends using the Gronlien article as a model. | Homework: Study dilution making for Lab 3; Generate two figures with legends from your Tetrahymena experiment: one or more microphotographs of Tetrahymena and a graph or table of data. | ||
1 | 3 | Feb. 7 to Feb. 11 |
Making Dilutions; Determination of toxicity of Heavy Metals on Tetrahymena; Design your own experiment exploring inhibitors and Tetrahymena phagocytosis |
Homework: There are animated tutorials on dilution available at the following link: Dilution Help Tutorials; Finalize your experimental design for Lab 4; have it approved by your lab instructor; Write a results section of a scientific report on your findings in the Tetrahymena toxicity experiment including a narrative portion and integrated figures with legends; Work the dilution problems found at Media: Lab_3_110_Dilution_Problems.doc and hand them in in Lab 4. | ||
1 | 4 | Feb. 14 to Feb. 18 |
Student Designed Experiments: Potential Inhibitors of Vacuole Formation; |
Homework: 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your Lab 4 experimental results. Read assigned journal article for science writing discussion * Gronlien, H.K., Berg, T., Lovlie, A.M. (2002). In the polymorphic ciliate Tetrahymena vorax, the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes, J. Exp. Biol. 205, 2089-2097 | ||
1 | 5 | Feb. 22 to Feb. 28 |
Scientific Writing Workshop with Journal Article Discussion; Science Writing Workshop; |
Homework: Tetrahymena Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6 Title Page, Results, Discussion and References sections. Guidelines at: Resources; Science Writing peer-tutor appointment scheduling at: Wellesley College Writing Program Resources. Have Lab notebooks ready to be turned in for inspection after Lab 6.
| ||
2 | 6 | March 1 to March 7 |
Genetics: Taster SNP 1-Test PTC Taster Phenotype; Isolate and digest DNA; PCR; |
Homework: Read the following background information on restriction enzymes and on gel electrophoresis Read the following background information on ethical issues in genetic testing: | ||
2 | 7 | March 8 to March 14 |
Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis; Determine PTC Taster Genotype; Scientific Writing Workshop |
Homework: Write a scientific research report (all sections except M&M)on your PTC Taster SNP study. Due on April 1st at 5pm. For help, see the Resources section of this wiki. | ||
3 | 8 | March 28 to April 1 |
Photosynthesis 1: Photosynthetic pigments | Homework: Prepare a full results section for the photosynthetic pigment identification experiment; In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9); | ||
3 | 9 | April 4 to April 8 |
Photosynthesis 2: Hill Reaction | Homework: Finalize your self-designed experiment testing a variable affecting the Hill Reaction; Write a Materials and Methods style version of the Hill Reaction experiment that you did this week in Lab 9 | ||
3 | 10 | April 11 to April 15 |
Photosynthesis 3: Investigation of variables affecting the Hill Reaction | Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments (with the treatments on the same graph).See(Appendix E). Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis); A full paper on your experiment, in the form of a scientific research report, | ||
4 | 11 | April 20 to April 26 |
Enzymology | Homework: Prepare for a Lab Practical Skills Assessment in lab 12. Answer the following questions (5 points) and hand them in to your instructor at the beginning of Lab 12. | ||
Skills Assessment | 12 | May 2 to May 6 |
Lab Practical; Turn in Lab Notebook |
End of lab |
Lab Graded Assignments
Lab Assigned | Lab Due | Assignment description |
Point Value (Total 200) |
---|---|---|---|
1 | 2 | Practice Problems | 5 |
2 | 3 | Fig. Design (ungraded) | 0 |
3 | 4 | Dilution Problems (5); Fig.& Results (10) |
15 |
4 | 5 | Oral presentation: Results, original figure & legend; Journal article discussion |
10 |
5 | 6 | Lab notebook check (ungraded); Partial Scientific Paper(25) |
25 |
6 | 7 | SNP Taster Questions | 5 |
7 | Due for all students Fri. 4/1 by 5pm |
Taster Scientific Paper (all but M&M) | 35 |
8 | 9 | Photosyn. Pigments Results | 10 |
9 | 10 | M&M (5pt); Finalize Self-Design Experiment Hill Rxn.(ungraded) |
5 |
10 | 11 | Full Scientific Paper Hill Rxn. | 35 |
11 | 12 | Lab Notebook Check (10) Concept Questions Enzymology/Gene Reg. (5) |
15 |
12 | 12 | Lab Practical:Hands-on skills assessment |
35 |
Other | - | Discretionary Points Preparation & Participation |
5 |