BISC 219/2009:Creating a Transgenic Organism: Difference between revisions
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##PCR to test directly for the presence of the introduced genes and <br> | ##PCR to test directly for the presence of the introduced genes and <br> | ||
#become adept at sterile technique. <br> | #become adept at sterile technique. <br> | ||
== Schedule of experiments == |
Revision as of 12:02, 13 April 2009
At the end of this semester long series you will have mastered the following skills and concepts using both microorganisms and a multicellular organism.
Experimental Objectives:
In this semester long experiment you will:
- use the Agrobacterium system to introduce a foreign gene, the β -glucuronidase gene (gusA) of E. coli, into the cells of tobacco
- take advantage of the totipotency of plant cells and use these transformed cells to regenerate genetically engineered plants
- confirm that the regenerated plants are transformed by
- measuring the activity of the introduced enzyme, β–glucuronidase (GUS) spectrophotometrically and histochemically
- PCR to test directly for the presence of the introduced genes and
- become adept at sterile technique.