BISC 219/2009:Gene Mapping: Difference between revisions
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[[BISC 219/2009: Mod 1 C. elegans General Information| ''C. elegans'' General Information]] <br> | [[BISC 219/2009: Mod 1 C. elegans General Information| ''C. elegans'' General Information]] <br> | ||
[[BISC 219/2009: Mod 1 Media and Tools | Tools and Techniques]]<br> | [[BISC 219/2009: Mod 1 Media and Tools | Tools and Techniques]]<br> | ||
[[BISC 219/2009: Mod 1 Lab 1 | Lab 1:]]<br> | [[BISC 219/2009: Mod 1 Lab 1 | Lab 1: Welcome to ''C. elegans'']]<br> | ||
[[BISC 219/2009: Mod 1 Lab 2 | Lab 2:]]<br> | [[BISC 219/2009: Mod 1 Lab 2 | Lab 2: Mutant Hunt!]]<br> | ||
[[BISC 219/2009: Mod 1 Lab 3 | Lab 3:]]<br> | [[BISC 219/2009: Mod 1 Lab 3 | Lab 3: Linkage Test and Backcross]]<br> | ||
[[BISC 219/2009: Mod 1 Lab 4 | Lab 4:]]<br> | [[BISC 219/2009: Mod 1 Lab 4 | Lab 4: Linkage Test, Backcross and Mapping]]<br> | ||
[[BISC 219/2009: Mod 1 Lab 5 | Lab 5:]]<br> | [[BISC 219/2009: Mod 1 Lab 5 | Lab 5: Mapping Part 2]]<br> | ||
[[BISC 219/2009: Mod 1 Lab 6 | Lab 6: Score!]]<br> | |||
=='''Schedule of Experiments'''== | =='''Schedule of Experiments'''== | ||
Revision as of 10:37, 24 June 2009
We would like to thank Dr. Phil Hartman of Texas Christian University for developing this series and providing us with the worms for our crosses.
C. elegans General Information
Tools and Techniques
Lab 1: Welcome to C. elegans
Lab 2: Mutant Hunt!
Lab 3: Linkage Test and Backcross
Lab 4: Linkage Test, Backcross and Mapping
Lab 5: Mapping Part 2
Lab 6: Score!
Schedule of Experiments
| Lab # | Dates | Activity | Outside lab time |
|---|---|---|---|
| 1 | 9/8 - 9/14 | view worm videos; examine wild type and mutant worms; |
3-4 days later: Pick 2 "wild type" looking worms from each cross to self |
| 2 | 9/15 - 9/21 | score autosomal vs X-linked cross; Mutant Hunt! Pick 3 worms to individual plates |
3-4 days later: cross wild type males by mutant hermaphrodite |
| 3 | 9/22 - 9/28 | Linkage Test: Cross mutant males by 4 tester strains; Backcross: Pick 2 "wild type" L4's to a new plate |
3-4 days later: Linkage Test: transfer 2 L4's from each cross to new plates |
