BISC 219/2009:Gene Mapping: Difference between revisions

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[[BISC 219/2009: Mod 1 C. elegans General Information| ''C. elegans'' General Information]] <br>
[[BISC 219/2009: Mod 1 C. elegans General Information| ''C. elegans'' General Information]] <br>
[[BISC 219/2009: Mod 1 Media and Tools | Tools and Techniques]]<br>
[[BISC 219/2009: Mod 1 Media and Tools | Tools and Techniques]]<br>
[[BISC 219/2009: Mod 1 Lab 1  | Lab 1: Welcome to ''C. elegans'']]<br>
[[BISC 219/2009: Mod 1 Lab 1  | Lab 1: Welcome to ''C. elegans'' and Mutant Hunt]]<br>
[[BISC 219/2009: Mod 1 Lab 2  | Lab 2: Mutant Hunt!]]<br>
[[BISC 219/2009: Mod 1 Lab 2  | Lab 2: Linkage Test and Backcross]]<br>
[[BISC 219/2009: Mod 1 Lab 3  | Lab 3: Linkage Test and Backcross]]<br>
[[BISC 219/2009: Mod 1 Lab 3 | Lab 3: Linkage Test and Backcross and Mapping]]<br>
[[BISC 219/2009: Mod 1 Lab 4 | Lab 4: Linkage Test, Backcross and Mapping]]<br>
[[BISC 219/2009: Mod 1 Lab 4 | Lab 4: Mapping Part 2]]<br>
[[BISC 219/2009: Mod 1 Lab 5 | Lab 5: Mapping Part 2]]<br>
[[BISC 219/2009: Mod 1 Lab 5 | Lab 5: Score!]]<br>
[[BISC 219/2009: Mod 1 Lab 6 | Lab 6: Score!]]<br>


=='''Schedule of Experiments'''==
=='''Schedule of Experiments'''==
Line 21: Line 20:
! 1
! 1
| 9/8 - 9/14
| 9/8 - 9/14
|'''Worm Husbandry''':view worm videos;  
|'''Worm Husbandry''': view worm videos; <br>
examine wild type and mutant worms; practice picking worms;<br>
<br>
Set up first crosses - '''Autosomal vs X-linked test'''
Examine wild type and mutant worms; practice picking worms;<br>
<br>
Set up first crosses - '''Autosomal vs X-linked test'''<br>
<br>
'''Mutant Hunt:'''  search "screen" plates for Dpy worms
|'''3-4 days later:'''<br>
|'''3-4 days later:'''<br>
Pick 2 "wild type" looking worms from each cross to self
Pick 2 "wild type" looking worms from each Autosomal vs X-linked cross to new plates<br>
<br>
Examine phenotypes of autosomal vs X-linked F1 generation<br>
<br>
Examine mutants from your hunt:  If Dpy then cross to N2 males
|-
|-
!2  
!2  
|9/15 - 9/21
|9/15 - 9/21
|Score autosomal vs X-linked cross;<br>
|Score autosomal vs X-linked cross;<br>
'''Mutant Hunt!''' Pick 3 worms to individual plates
'''Linkage Test:''' Cross mutant males by 4 tester strains;<br>
|'''3-4 days later:'''<br>
|'''3-4 days later:'''<br>
cross wild type males by mutant hermaphrodite
'''Linkage Test:''' transfer 2 L4's from each cross to new plates (4 plates total)<br>
|-
|-
!3
!3
|9/22 - 9/28  
|9/22 - 9/28
|'''Linkage Test:''' Cross mutant males by 4 tester strains;<br>
|'''Linkage Test:'''Score for linkage<br>
'''Backcross:''' Pick 2 "wild type" L4's to a new plate
'''Mapping:'''Pick 5 Dumpy worms to individual plates
|'''3-4 days later:'''<br>
|'''3-4 days later:'''<br>
'''Linkage Test:''' transfer 2 L4's from each cross to new plates<br>
'''Mapping:''' Pick 3 double mutants (unc and dyp) to separate plates<br>
'''Backcross:''' transfer 2 L4's to a new plate
'''Complementation:''' Cross your unknown Dpy mutant worms by N2 males
|-
|-
!4
!4
|9/29 - 10/5
|9/29 - 10/5
|'''Linkage Test:'''Score for linkage<br>
|'''Mapping:''' cross N2 males with double mutants<br>
'''Backcross:'''confirm true breeding line<br>
'''Complementation:''' Pick males from Complementation cross #1 and mate with known Dpy strains
'''Mapping:'''Pick 5 uncs to individual plates  
|'''3-4 days later:''' <br>
'''Mapping:''' Pick 3-4 heterozygous males to separate plates (8 males total) and mate with homozygous recessive dpy unc worms<br>
'''Complementation:''' Examine cross plates for Dpy males
|-
!5
|10/6 - 10-14
|'''Mapping:''' Score and determine map distance between unknown Dpy gene and Unc reference mutation
|
|
|-
|-
|-

Latest revision as of 08:26, 28 September 2009

Wellesley College BISC 219 Genetics

We would like to thank Dr. Phil Hartman of Texas Christian University for developing this series and providing us with the worms for our crosses.

C. elegans General Information
Tools and Techniques
Lab 1: Welcome to C. elegans and Mutant Hunt
Lab 2: Linkage Test and Backcross
Lab 3: Linkage Test and Backcross and Mapping
Lab 4: Mapping Part 2
Lab 5: Score!

Schedule of Experiments

Lab # Dates Activity Outside lab time
1 9/8 - 9/14 Worm Husbandry: view worm videos;


Examine wild type and mutant worms; practice picking worms;

Set up first crosses - Autosomal vs X-linked test

Mutant Hunt: search "screen" plates for Dpy worms

3-4 days later:

Pick 2 "wild type" looking worms from each Autosomal vs X-linked cross to new plates

Examine phenotypes of autosomal vs X-linked F1 generation

Examine mutants from your hunt: If Dpy then cross to N2 males

2 9/15 - 9/21 Score autosomal vs X-linked cross;

Linkage Test: Cross mutant males by 4 tester strains;

 3-4 days later:

Linkage Test: transfer 2 L4's from each cross to new plates (4 plates total)

3 9/22 - 9/28 Linkage Test:Score for linkage

Mapping:Pick 5 Dumpy worms to individual plates

3-4 days later:

Mapping: Pick 3 double mutants (unc and dyp) to separate plates
Complementation: Cross your unknown Dpy mutant worms by N2 males

4 9/29 - 10/5 Mapping: cross N2 males with double mutants

Complementation: Pick males from Complementation cross #1 and mate with known Dpy strains

3-4 days later:

Mapping: Pick 3-4 heterozygous males to separate plates (8 males total) and mate with homozygous recessive dpy unc worms
Complementation: Examine cross plates for Dpy males

5 10/6 - 10-14 Mapping: Score and determine map distance between unknown Dpy gene and Unc reference mutation
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