BISC 219/2009:Gene Mapping
We would like to thank Dr. Phil Hartman of Texas Christian University for developing this series and providing us with the worms for our crosses.
C. elegans General Information
Tools and Techniques
Lab 1: Welcome to C. elegans
Lab 2: Mutant Hunt!
Lab 3: Linkage Test and Backcross
Lab 4: Linkage Test, Backcross and Mapping
Lab 5: Mapping Part 2
Lab 6: Score!
Schedule of Experiments
| Lab # | Dates | Activity | Outside lab time |
|---|---|---|---|
| 1 | 9/8 - 9/14 | view worm videos; examine wild type and mutant worms; |
3-4 days later: Pick 2 "wild type" looking worms from each cross to self |
| 2 | 9/15 - 9/21 | score autosomal vs X-linked cross; Mutant Hunt! Pick 3 worms to individual plates |
3-4 days later: cross wild type males by mutant hermaphrodite |
| 3 | 9/22 - 9/28 | Linkage Test: Cross mutant males by 4 tester strains; Backcross: Pick 2 "wild type" L4's to a new plate |
3-4 days later: Linkage Test: transfer 2 L4's from each cross to new plates |
| 4 | 9/29 - 10/5 | Linkage Test:Score for linkage Backcross:confirm true breeding line |
