BISC 219/2009:Gene Mapping
We would like to thank Dr. Phil Hartman of Texas Christian University for developing this series and providing us with the worms for our crosses.
C. elegans General Information
Tools and Techniques
Lab 1: Welcome to C. elegans and Mutant Hunt
Lab 2: Linkage Test and Backcross
Lab 3: Linkage Test and Backcross and Mapping
Lab 4: Mapping Part 2
Lab 5: Score!
Schedule of Experiments
Lab # | Dates | Activity | Outside lab time |
---|---|---|---|
1 | 9/8 - 9/14 | Worm Husbandry: view worm videos;
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3-4 days later: Pick 2 "wild type" looking worms from each Autosomal vs X-linked cross to new plates |
2 | 9/15 - 9/21 | Score autosomal vs X-linked cross; Linkage Test: Cross mutant males by 4 tester strains; |
3-4 days later: Linkage Test: transfer 2 L4's from each cross to new plates (4 plates total) |
3 | 9/22 - 9/28 | Linkage Test:Score for linkage Mapping:Pick 5 Dpy worms to individual plates |
3-4 days later: Mapping: Pick 3 double mutants to separate plates |
4 | 9/29 - 10/5 | Mapping: cross N2 males with double mutants Complementation: Pick males from Complementation cross #1 and mate with known Dpy strains |
3-4 days later: Mapping: Pick 2 heterozygotes to separate plates |
5 | 10/6 - 10-14 | Mapping: Score and determine map distance between unknown Dpy gene and Unc reference mutation |