BISC 219/F10 - Revision history

Tucker Crum: /* Fall 2010 */

Tucker Crum — Thu, 28 Oct 2010 16:49:39 GMT

Fall 2010

←Older revision		Revision as of 16:49, 28 October 2010
Line 25:		Line 25:
	[http://www.wellesley.edu/ScienceCenter/Safety/safety_index.html Wellesley Safety]. This site has specific safety information related to the type of experiments you will perform. There are times when you should wear gloves in lab to prevent contaminating your samples and also to prevent your samples from contaminating you. Please remember, however, that your gloves can be easily contaminated if you touch your face, hair, etc. and that you can contaminate door-knobs, computer keyboards, phones or other non-lab equipment with your gloves. Change your gloves often and never touch non-lab equipment (such as computer key boards) with gloved hands unless specifically directed to do so.<br>		[http://www.wellesley.edu/ScienceCenter/Safety/safety_index.html Wellesley Safety]. This site has specific safety information related to the type of experiments you will perform. There are times when you should wear gloves in lab to prevent contaminating your samples and also to prevent your samples from contaminating you. Please remember, however, that your gloves can be easily contaminated if you touch your face, hair, etc. and that you can contaminate door-knobs, computer keyboards, phones or other non-lab equipment with your gloves. Change your gloves often and never touch non-lab equipment (such as computer key boards) with gloved hands unless specifically directed to do so.<br>
	<br>		<br>
-	Be forewarned that you ~~will~~ be expected to come into lab outside of normal hours to collect data or to continue an experiment. We have tried to keep the extra time to a minimum, but please understand that BISC219 lab is an ambitious program in genetics requiring your full commitment and cooperation. <br>	+	Be forewarned that you are expected to attend lab every week and you may be expected to come into lab outside of normal hours to collect data or to continue an experiment. We have tried to keep the extra time to a minimum, but please understand that BISC219 lab is an ambitious program in genetics requiring your full commitment and cooperation. <br>
	<br>		<br>
	The three series of experiments you will perform during the semester are:<br>		The three series of experiments you will perform during the semester are:<br>

Tucker Crum: /* Links to Labs& Project Info */

Tucker Crum — Fri, 01 Oct 2010 01:49:51 GMT

Links to Labs& Project Info

←Older revision		Revision as of 01:49, 1 October 2010
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	[[BISC 219/F10: Lab 3 \| Lab 3: Linkage Test Part 1]]<br>		[[BISC 219/F10: Lab 3 \| Lab 3: Linkage Test Part 1]]<br>
	[[BISC 219/F10: Lab 4 \| Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>		[[BISC 219/F10: Lab 4 \| Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>
-	[[BISC 219/F10: Lab 5 \| Lab 5: Mapping Con't]]<br>	+	[[BISC 219/F10: Lab 5 \| Lab 5: Finish Complementation; Mapping Con't]]<br>
-	[[BISC 219/F10: Lab 6 \| Lab 6: ~~Finish Complementation~~; Mapping Con't]]<BR>	+	[[BISC 219/F10: Lab 6 \| Lab 6: DNA sequence analysis; Mapping Con't]]<BR>
	[[BISC 219/F10: Lab 7 \| Lab 7: Complete Mapping: Score]]<br>		[[BISC 219/F10: Lab 7 \| Lab 7: Complete Mapping: Score]]<br>
	Series3:<BR>		Series3:<BR>

Tucker Crum: /* Links to Labs& Project Info */

Tucker Crum — Fri, 01 Oct 2010 00:34:46 GMT

Links to Labs& Project Info

←Older revision		Revision as of 00:34, 1 October 2010
Line 53:		Line 53:
	[[BISC 219/F10: Lab 3 \| Lab 3: Linkage Test Part 1]]<br>		[[BISC 219/F10: Lab 3 \| Lab 3: Linkage Test Part 1]]<br>
	[[BISC 219/F10: Lab 4 \| Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>		[[BISC 219/F10: Lab 4 \| Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>
-	[[BISC 219/F10: Lab 5 \| Lab 5: Mapping ~~Part 2~~]]<br>	+	[[BISC 219/F10: Lab 5 \| Lab 5: Mapping Con't]]<br>
-	[[BISC 219/F10: Lab 6 \| Lab 6: Score ~~& DNA sequencing analysis~~]]<br>	+	[[BISC 219/F10: Lab 6 \| Lab 6: Finish Complementation; Mapping Con't]]<BR>
		+	[[BISC 219/F10: Lab 7 \| Lab 7: Complete Mapping: Score]]<br>
	Series3:<BR>		Series3:<BR>
	[[BISC 219/F10:RNA interference \| Schedule of Reverse Genetics Project]]<BR>		[[BISC 219/F10:RNA interference \| Schedule of Reverse Genetics Project]]<BR>

Tucker Crum: /* Fall 2010 */

Tucker Crum — Fri, 03 Sep 2010 19:07:41 GMT

Fall 2010

←Older revision		Revision as of 19:07, 3 September 2010
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	<br>		<br>
	We sincerely hope that you find your introduction to the world of genetics both exciting and intellectually stimulating. As always, we appreciate comments and suggestions for improving the program. Good luck and have fun!		We sincerely hope that you find your introduction to the world of genetics both exciting and intellectually stimulating. As always, we appreciate comments and suggestions for improving the program. Good luck and have fun!
		+
		+	==Links to Labs& Project Info==
		+	Series1:<BR>
		+	[[BISC 219/F10: Worm Info\| Worm Info]] <br>
		+	[[BISC 219/F10: Gene Linkage\| Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start]]<BR>
		+	[[BISC 219/F10: Lab 2 \| Lab 2: Sex-Linked or Autosomal Finale]]<br>
		+	Series2:<BR>
		+	[[BISC 219/F10: Gene Mapping Info \| Background: Classical Forward Genetics and Gene Mapping]]<br>
		+	[[BISC 219/F10: Lab 2 Mutant Hunt \| Lab 2: Mutant Hunt]]<br>
		+	[[BISC 219/F10: Lab 3 \| Lab 3: Linkage Test Part 1]]<br>
		+	[[BISC 219/F10: Lab 4 \| Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>
		+	[[BISC 219/F10: Lab 5 \| Lab 5: Mapping Part 2]]<br>
		+	[[BISC 219/F10: Lab 6 \| Lab 6: Score & DNA sequencing analysis]]<br>
		+	Series3:<BR>
		+	[[BISC 219/F10:RNA interference \| Schedule of Reverse Genetics Project]]<BR>
		+	[[BISC 219/F10:RNAi General Information\| RNAi General Information]] <br>
		+	[[BISC 219/F10:Media Recipes \| Media Recipes]]<br>
		+	[[BISC 219/F10: RNAi Lab 5 \| Lab 5: Picking your gene to RNAi]]<br>
		+	[[BISC 219/F10: RNAi Lab 6 \| Lab 6: Cloning your gene of interest]]<br>
		+	[[BISC 219/F10: RNAi Lab 7 \| Lab 7: Picking your transformant]]<br>
		+	[[BISC 219/F10: RNAi Lab 8 \| Lab 8: Plasmid purification and transformation]]<br>
		+	[[BISC 219/F10: RNAi Lab 9 \| Lab 9: Induction of bacteria for RNAi]]<br>
		+	[[BISC 219/F10: RNAi Lab 10 \| Lab 10: Scoring your worms and RNA purification]]<br>
		+	[[BISC 219/F10: RNAi Lab 11 \| Lab 11: RT PCR reactions]]<br><br>

Tucker Crum: /* Fall 2010 */

Tucker Crum — Tue, 31 Aug 2010 15:16:41 GMT

Fall 2010

←Older revision		Revision as of 15:16, 31 August 2010
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	'''Welcome to BISC 219!'''<br>		'''Welcome to BISC 219!'''<br>
	Please complete two brief surveys BEFORE attending your first lab and lecture about interest and concept familiarity with genetics. These surveys are found at: [http://www.surveymonkey.com/s/5XPB32W] and at [http://www.surveymonkey.com/s/TYS8CTH] (http://www.surveymonkey.com/s/5XPB32W and at http://www.surveymonkey.com/s/TYS8CTH)<BR>		Please complete two brief surveys BEFORE attending your first lab and lecture about interest and concept familiarity with genetics. These surveys are found at: [http://www.surveymonkey.com/s/5XPB32W] and at [http://www.surveymonkey.com/s/TYS8CTH] (http://www.surveymonkey.com/s/5XPB32W and at http://www.surveymonkey.com/s/TYS8CTH)<BR>
-	The password for both surveys is posted to the welcome email message in your First Class Lab conference. Thank you for your participation. You will get a few bonus points at the end of the semester for completing the entry and the exit versions of these surveys.<BR><BR>	+	The password for both surveys is posted to the welcome email message in your First Class Lab conference. Thank you for your participation. You will get a few bonus points at the end of the semester for completing the entry and the exit versions of these surveys.<BR><BR>

	Over the course of the semester you will perform experiments that encompass both forward and reverse genetics approaches to answer complex questions about gene structure and function using the nematode worm, ''Caenorhabditis elegans'', as a model organism. You will gain familiarity with sophisticated molecular tools that are widely used to study gene location, regulation and expression. <br>		Over the course of the semester you will perform experiments that encompass both forward and reverse genetics approaches to answer complex questions about gene structure and function using the nematode worm, ''Caenorhabditis elegans'', as a model organism. You will gain familiarity with sophisticated molecular tools that are widely used to study gene location, regulation and expression. <br>

Tucker Crum: /* Fall 2010 */

Tucker Crum — Tue, 31 Aug 2010 15:16:08 GMT

Fall 2010

←Older revision		Revision as of 15:16, 31 August 2010
Line 13:		Line 13:

	'''Welcome to BISC 219!'''<br>		'''Welcome to BISC 219!'''<br>
-	Please complete two brief surveys BEFORE attending your first lab and lecture about interest and concept familiarity with genetics. These surveys are found at: [http://www.surveymonkey.com/s/5XPB32W and at http://www.surveymonkey.com/s/TYS8CTH \| http://www.surveymonkey.com/s/5XPB32W and at http://www.surveymonkey.com/s/TYS8CTH]<BR>	+	Please complete two brief surveys BEFORE attending your first lab and lecture about interest and concept familiarity with genetics. These surveys are found at: [http://www.surveymonkey.com/s/5XPB32W] and at [http://www.surveymonkey.com/s/TYS8CTH] (http://www.surveymonkey.com/s/5XPB32W and at http://www.surveymonkey.com/s/TYS8CTH)<BR>
	The password for both surveys is posted to the welcome email message in your First Class Lab conference. Thank you for your participation. You will get a few bonus points at the end of the semester for completing the entry and the exit versions of these surveys.<BR><BR>		The password for both surveys is posted to the welcome email message in your First Class Lab conference. Thank you for your participation. You will get a few bonus points at the end of the semester for completing the entry and the exit versions of these surveys.<BR><BR>

Tucker Crum: /* Fall 2010 */

Tucker Crum — Tue, 31 Aug 2010 15:14:33 GMT

Fall 2010

←Older revision		Revision as of 15:14, 31 August 2010
Line 13:		Line 13:

	'''Welcome to BISC 219!'''<br>		'''Welcome to BISC 219!'''<br>
-	Please complete two brief surveys BEFORE attending your first lab and lecture about interest and concept familiarity with genetics. These surveys are found at: ~~\| Click here to take the BISC219 entry LAB survey~~ at http://www.surveymonkey.com/s/5XPB32W and at http://www.surveymonkey.com/s/ The password for both is posted to the welcome email message in your First Class Lab conference.	+	Please complete two brief surveys BEFORE attending your first lab and lecture about interest and concept familiarity with genetics. These surveys are found at: [http://www.surveymonkey.com/s/5XPB32W and at http://www.surveymonkey.com/s/TYS8CTH \| http://www.surveymonkey.com/s/5XPB32W and at http://www.surveymonkey.com/s/TYS8CTH]<BR>
-		+	The password for both surveys is posted to the welcome email message in your First Class Lab conference. Thank you for your participation. You will get a few bonus points at the end of the semester for completing the entry and the exit versions of these surveys.<BR><BR>

	Over the course of the semester you will perform experiments that encompass both forward and reverse genetics approaches to answer complex questions about gene structure and function using the nematode worm, ''Caenorhabditis elegans'', as a model organism. You will gain familiarity with sophisticated molecular tools that are widely used to study gene location, regulation and expression. <br>		Over the course of the semester you will perform experiments that encompass both forward and reverse genetics approaches to answer complex questions about gene structure and function using the nematode worm, ''Caenorhabditis elegans'', as a model organism. You will gain familiarity with sophisticated molecular tools that are widely used to study gene location, regulation and expression. <br>

Tucker Crum: /* Fall 2010 */

Tucker Crum — Tue, 31 Aug 2010 15:10:42 GMT

Fall 2010

←Older revision		Revision as of 15:10, 31 August 2010
Line 13:		Line 13:

	'''Welcome to BISC 219!'''<br>		'''Welcome to BISC 219!'''<br>
		+	Please complete two brief surveys BEFORE attending your first lab and lecture about interest and concept familiarity with genetics. These surveys are found at: \| Click here to take the BISC219 entry LAB survey at http://www.surveymonkey.com/s/5XPB32W and at http://www.surveymonkey.com/s/ The password for both is posted to the welcome email message in your First Class Lab conference.
		+

	Over the course of the semester you will perform experiments that encompass both forward and reverse genetics approaches to answer complex questions about gene structure and function using the nematode worm, ''Caenorhabditis elegans'', as a model organism. You will gain familiarity with sophisticated molecular tools that are widely used to study gene location, regulation and expression. <br>		Over the course of the semester you will perform experiments that encompass both forward and reverse genetics approaches to answer complex questions about gene structure and function using the nematode worm, ''Caenorhabditis elegans'', as a model organism. You will gain familiarity with sophisticated molecular tools that are widely used to study gene location, regulation and expression. <br>

Tucker Crum: /* Fall 2010 */

Tucker Crum — Fri, 20 Aug 2010 12:49:55 GMT

Fall 2010

←Older revision		Revision as of 12:49, 20 August 2010
Line 20:		Line 20:
	Many of the reagents used in these experiments are expensive and they require many hours of preparation. In using them, please exercise care and common sense to avoid contaminating reagent stocks and to make sure you don’t “hord” shared reagents. A considerable amount of money has been spent to provide enough equipment of adequate sophistication so that each student will have “hands-on” experience. For example, you will be using micropipets for dispensing tiny quantities of solution very accurately. These micropipets cost approximately $250 each. Please treat all of the equipment with care so that many classes of genetics students can use them. <br>		Many of the reagents used in these experiments are expensive and they require many hours of preparation. In using them, please exercise care and common sense to avoid contaminating reagent stocks and to make sure you don’t “hord” shared reagents. A considerable amount of money has been spent to provide enough equipment of adequate sophistication so that each student will have “hands-on” experience. For example, you will be using micropipets for dispensing tiny quantities of solution very accurately. These micropipets cost approximately $250 each. Please treat all of the equipment with care so that many classes of genetics students can use them. <br>
	<br>		<br>
-	~~At times you~~ will be required to follow specific instructions concerning the handling or disposal of materials. Please obey these instructions precisely, both for your own protection and to comply with established guidelines for use of these reagents. '''You should read the Wellesley College Safety Information at:'''	+	You will be required to follow specific instructions concerning the handling or disposal of materials. Please obey these instructions precisely, both for your own protection and to comply with established guidelines for use of these reagents. '''You should read the Wellesley College Safety Information at:'''
	[http://www.wellesley.edu/ScienceCenter/Safety/safety_index.html Wellesley Safety]. This site has specific safety information related to the type of experiments you will perform. There are times when you should wear gloves in lab to prevent contaminating your samples and also to prevent your samples from contaminating you. Please remember, however, that your gloves can be easily contaminated if you touch your face, hair, etc. and that you can contaminate door-knobs, computer keyboards, phones or other non-lab equipment with your gloves. Change your gloves often and never touch non-lab equipment (such as computer key boards) with gloved hands unless specifically directed to do so.<br>		[http://www.wellesley.edu/ScienceCenter/Safety/safety_index.html Wellesley Safety]. This site has specific safety information related to the type of experiments you will perform. There are times when you should wear gloves in lab to prevent contaminating your samples and also to prevent your samples from contaminating you. Please remember, however, that your gloves can be easily contaminated if you touch your face, hair, etc. and that you can contaminate door-knobs, computer keyboards, phones or other non-lab equipment with your gloves. Change your gloves often and never touch non-lab equipment (such as computer key boards) with gloved hands unless specifically directed to do so.<br>
	<br>		<br>
-	~~On occasion,~~ you will be expected to come into lab outside of normal hours to collect data or to continue an experiment. We have tried to keep the extra time to a minimum, but please understand that BISC219 lab is an ambitious program in genetics requiring your full commitment and cooperation. <br>	+	Be forewarned that you will be expected to come into lab outside of normal hours to collect data or to continue an experiment. We have tried to keep the extra time to a minimum, but please understand that BISC219 lab is an ambitious program in genetics requiring your full commitment and cooperation. <br>
	<br>		<br>
	The three series of experiments you will perform during the semester are:<br>		The three series of experiments you will perform during the semester are:<br>

Tucker Crum: /* Fall 2010 */

Tucker Crum — Fri, 20 Aug 2010 12:47:18 GMT

Fall 2010

←Older revision		Revision as of 12:47, 20 August 2010
Line 28:		Line 28:
	'''Series 1:''' Autosome or Sex Chromosome?		'''Series 1:''' Autosome or Sex Chromosome?

-	'''Series 2:''' ~~Mapping~~ a Mutation	+	'''Series 2:''' Classical (Forward) Genetics: Locating & Characterizing a Gene Mutation Associated with a Abnormal Phenotype in ''C. elegans''

-	'''Series 3:''' RNA interference (RNAi)	+	'''Series 3:''' Reverse Genetics using RNA interference (RNAi)
	<br>		<br>
	<br>		<br>

←Older revision		Revision as of 01:49, 1 October 2010
Line 53:		Line 53:
	[[BISC 219/F10: Lab 3 \| Lab 3: Linkage Test Part 1]]<br>		[[BISC 219/F10: Lab 3 \| Lab 3: Linkage Test Part 1]]<br>
	[[BISC 219/F10: Lab 4 \| Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>		[[BISC 219/F10: Lab 4 \| Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>
-	[[BISC 219/F10: Lab 5 \| Lab 5: Mapping Con't]]<br>	+	[[BISC 219/F10: Lab 5 \| Lab 5: Finish Complementation; Mapping Con't]]<br>
-	[[BISC 219/F10: Lab 6 \| Lab 6: ~~Finish Complementation~~; Mapping Con't]]<BR>	+	[[BISC 219/F10: Lab 6 \| Lab 6: DNA sequence analysis; Mapping Con't]]<BR>
	[[BISC 219/F10: Lab 7 \| Lab 7: Complete Mapping: Score]]<br>		[[BISC 219/F10: Lab 7 \| Lab 7: Complete Mapping: Score]]<br>
	Series3:<BR>		Series3:<BR>

←Older revision		Revision as of 00:34, 1 October 2010
Line 53:		Line 53:
	[[BISC 219/F10: Lab 3 \| Lab 3: Linkage Test Part 1]]<br>		[[BISC 219/F10: Lab 3 \| Lab 3: Linkage Test Part 1]]<br>
	[[BISC 219/F10: Lab 4 \| Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>		[[BISC 219/F10: Lab 4 \| Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>
-	[[BISC 219/F10: Lab 5 \| Lab 5: Mapping ~~Part 2~~]]<br>	+	[[BISC 219/F10: Lab 5 \| Lab 5: Mapping Con't]]<br>
-	[[BISC 219/F10: Lab 6 \| Lab 6: Score ~~& DNA sequencing analysis~~]]<br>	+	[[BISC 219/F10: Lab 6 \| Lab 6: Finish Complementation; Mapping Con't]]<BR>
		+	[[BISC 219/F10: Lab 7 \| Lab 7: Complete Mapping: Score]]<br>
	Series3:<BR>		Series3:<BR>
	[[BISC 219/F10:RNA interference \| Schedule of Reverse Genetics Project]]<BR>		[[BISC 219/F10:RNA interference \| Schedule of Reverse Genetics Project]]<BR>

←Older revision		Revision as of 19:07, 3 September 2010
Line 42:		Line 42:
	<br>		<br>
	We sincerely hope that you find your introduction to the world of genetics both exciting and intellectually stimulating. As always, we appreciate comments and suggestions for improving the program. Good luck and have fun!		We sincerely hope that you find your introduction to the world of genetics both exciting and intellectually stimulating. As always, we appreciate comments and suggestions for improving the program. Good luck and have fun!
		+
		+	==Links to Labs& Project Info==
		+	Series1:<BR>
		+	[[BISC 219/F10: Worm Info\| Worm Info]] <br>
		+	[[BISC 219/F10: Gene Linkage\| Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start]]<BR>
		+	[[BISC 219/F10: Lab 2 \| Lab 2: Sex-Linked or Autosomal Finale]]<br>
		+	Series2:<BR>
		+	[[BISC 219/F10: Gene Mapping Info \| Background: Classical Forward Genetics and Gene Mapping]]<br>
		+	[[BISC 219/F10: Lab 2 Mutant Hunt \| Lab 2: Mutant Hunt]]<br>
		+	[[BISC 219/F10: Lab 3 \| Lab 3: Linkage Test Part 1]]<br>
		+	[[BISC 219/F10: Lab 4 \| Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>
		+	[[BISC 219/F10: Lab 5 \| Lab 5: Mapping Part 2]]<br>
		+	[[BISC 219/F10: Lab 6 \| Lab 6: Score & DNA sequencing analysis]]<br>
		+	Series3:<BR>
		+	[[BISC 219/F10:RNA interference \| Schedule of Reverse Genetics Project]]<BR>
		+	[[BISC 219/F10:RNAi General Information\| RNAi General Information]] <br>
		+	[[BISC 219/F10:Media Recipes \| Media Recipes]]<br>
		+	[[BISC 219/F10: RNAi Lab 5 \| Lab 5: Picking your gene to RNAi]]<br>
		+	[[BISC 219/F10: RNAi Lab 6 \| Lab 6: Cloning your gene of interest]]<br>
		+	[[BISC 219/F10: RNAi Lab 7 \| Lab 7: Picking your transformant]]<br>
		+	[[BISC 219/F10: RNAi Lab 8 \| Lab 8: Plasmid purification and transformation]]<br>
		+	[[BISC 219/F10: RNAi Lab 9 \| Lab 9: Induction of bacteria for RNAi]]<br>
		+	[[BISC 219/F10: RNAi Lab 10 \| Lab 10: Scoring your worms and RNA purification]]<br>
		+	[[BISC 219/F10: RNAi Lab 11 \| Lab 11: RT PCR reactions]]<br><br>