| Lab Assigned || Lab Due || Assignment|
| Point Value |
| Lab 1
|| Lab 2
|| Complete entry assessment (2.5pts); Submit preliminary cross results Day 3 (1 pt);|
Summarize experimental question, design; Diagram crosses; Explain data interpretation (9 pts) BISC_219/F10: Assignment_1_Lab1 ;
Read the following journal article and be prepared to discuss the questions in Lab3 found at :BISC_219:Questions to Guide Your Reading 1.
| Lab 2
|| Lab 3
|| Statement of Series 2 experimental question & goals. Outline of experimental process including : Mutant Hunt, Linkage Analysis, Mapping the Mutation to a chromosome and to a particular location, Complementation Analysis, Determining whether or not the mutation has been previously characterized, & Defining the gene sequence and protein change through DNA sequence analysis. 1-2 page summary of how you will determine the exact location of the gene and the sequence change likely to be associated with a phenotypic anomally.BISC_219/F10: Assignment_Series2_Outline_Summary
| Lab 2
|| Lab 4
BISC 219/F10: Assignment Help- Data Analysis 1
Autosomal vs. Sex linked inheritance
| Lab 3
|| Lab 4
|| Diagram the expected results (genotype and phenotype)for the crosses and self-fertilization progeny in the Linkage Testing work that you will do over the next several weeks. Highlight the progeny that will be used to continue each step of the process. Include a narrative description of the rational for the crosses and summarize the interpretation of the results that will allow determination of the linkage group to which your mutated gene of interest belongs. Assignment information and rubric found at BISC_219/F10: Assignment_Series2_Linkage Testing Crosses
| Lab 4
|| Lab 5
|| Diagram the expected results (genotype and phenotype)for the crosses and self-fertilization progeny in the Mapping work that you will do over the next several weeks. Make sure you highlight the progeny that will be used to continue each step of the process.
| Lab 5
|| Lab 6
|| Explain complementation analysis in general and then explain specifically how you will use it to confirm and expand your linkage and mapping work. Wikipedia has a good general introduction at
| Lab 6
|| Lab 7
|| Analyze your mutant gene's DNA sequence and write a 1-2 page summary describing briefly and generally how chain-termination sequencing is accomplished and then specifically describe the mutation most likely to be responsible for your phenotype change by comparing the wild type sequence to the mutant's gene. Include the resulting protein or regulatory product change and its likely significance in the worm and, if possible, in other species.
| Lab 6
|| Week of Nov. 1
|| Scientific Research Report on Series2 work Classical (Forward) Genetics. Assignment information found at BISC 219/F10: Assignment_ Series2_Classical Genetics Paper|
| Lab 7
|| Week of Nov. 1
|| Work on Series2 Paper
| Lab 8
|| Lab 9
|| Convert all the lab protocols performed so far for Series 3: Reverse Genetics Using RNAi to M&M. Assignment information found at: BISC_219/F10: Assignment_Series3_ Materials and Methods; |
Read the original 1998 paper by Fire and Mello published in Nature; read a later review by Yigit et.al, DOI 10.1016/j.cell.2006.09.033, and the associated perspective,DOI 10.1016/j.cell.2006.11.004, both published in Cell; also read a recent article by Trujillo et.alin Genetics,DOI10.1534/genetics.110.117341, that uses RNAi and other genetic tools that you have learned about in the lab and lecture sections of this course. There are questions to guide your reading found at:BISC_219:Questions to Guide Your Reading 2 . Please be prepared to discuss those questions in Lab 9.
| Lab 9
|| Lab 10
|| Write a draft introduction section (including properly formatted Literature Cited page) of your next paper on our Reverse Genetics Project. Refer to BISC_219/F10:Resources Guide to Scientific Writing.
| Lab 10
|| Nov. 24 |
for all students
| Construct figures/tables with properly formatted legends to illustrate the main findings of the RNAi part of your Series3:Reverse Genetics project. These figures are a draft of those that will be part of the results section of the research report you will write for Series3.
| Lab 11
|| Lab 12
|| Full scientific research report due Fri. Dec 10 at 4pm for all students. Assignment information found at BISC_219/F10: Assignment_Series3_Reverse Genetics Paper using RNAi
|| Exit Survey |