BISC 219/F10:Calendars/Planner

BISC219 F10 Lab Calendar

Monday	Tuesday	Wednesday	Thursday	Friday
Sept. 6 Labor Day Holiday	Sept. 7 Lab 1	Sept. 8 Lab 1	Sept. 9 Lab 1	Sept. 10 Lab 1
Sept. 13 Lab 1	Sept. 14 Lab 2	Sept. 15 Lab 2	Sept. 16 Lab 2	Sept. 17 Lab 2
Sept. 20 Lab 2	Sept. 21 Lab 3	Sept. 22 Lab 3	Sept. 23 Lab 3	Sept. 24 Lab 3
Sept. 27 Lab 3	Sept. 28 Lab 4	Sept. 29 Lab 4	Sept. 30 Lab 4	Oct. 1 Lab 4
Oct. 4 Lab 4	Oct. 5 Lab 5	Oct. 6 Lab 5	Oct. 7 Lab 5	Oct. 8 Lab 5
Oct. 11 Fall Break No Lab	Oct. 12 MON. Sch. Lab 5	Oct. 13 Lab 6	Oct. 14 Lab 6	Oct. 15 Lab 6
Oct. 18 Lab 6	Oct. 19 Lab 6	Oct. 20 Lab 7	Oct. 21 Lab 7	Oct. 22 Lab 7
Oct. 25 Lab 7	Oct. 26 Lab 7	Oct. 27 Tanner Conf. No Lab	Oct. 28 Lab 8	Oct. 29 Lab 8
Nov. 1 Lab 8	Nov. 2 Lab 8	Nov. 3 Lab 8	Nov. 4 Lab 9	Nov. 5 Lab 9
Nov. 8 Lab 9	Nov. 9 Lab 9	Nov. 10 Lab 9	Nov. 11 Lab 10	Nov. 12 Lab 10
Nov. 15 Lab 10	Nov. 16 Lab 10	Nov. 17 Lab 10	Nov. 18 NO Lab	Nov. 19 No Lab
Nov. 22 No Lab	Nov. 23 No Lab	Nov. 24 No Lab	Nov. 25 Thanksgiving Holiday	Nov. 26 Thanksgiving Holiday
Nov. 29 Lab 11	Nov. 30 Lab 11	Dec. 1 Lab 11	Dec. 2 Lab 11	Dec. 3 Lab 11
Dec. 6 Lab 12	Dec. 7 Lab 12	Dec. 8 Lab 12	Dec. 9 Lab 12	Dec. 10 Lab 12

Schedule of Experiments

Series	Title	Lab #
1	Worm Boot Camp; Autosomal and Sex-linked Traits	1-2
2	Gene Mapping	2-6
3	RNAi	7-12

Graded Assignments

Lab Assigned	Lab Due	Assignment description	Point Value (Total 200)
Lab 1	Lab 2	Complete entry survey (2.5pts); Submit preliminary cross results Day 3; Summarize experimental question, design; Diagram crosses; Explain data interpretation (10pts)	12.5
Lab 2	Lab 3	Results Section BISC 219/F10: Assignment Help- Data Analysis 1 Autosomal vs. Sex linked inheritance	30
Lab 3	Lab 4
Lab 4	Lab 5
Lab 5	Lab 6
Lab 6	Lab 7
Lab 7	Lab 8
Lab 8	Lab 9
Lab 9	Lab 10
Lab 10	Lab 11
Lab 11	Lab 12
Other		Discretionary Points: Preparation & Participation	5

BISC219 F10 Weekly Lab Planner

Lab	Date	In-Lab Work	Outside of Lab Work	Assignment
1	Sept. 7 to Sept. 13	LEARN WORM HUSBANDRY; View worm videos; Make your worm pick; Examine worms: recognize different stages & sexes & differentiate mutations; Practice picking worms; Start Series 1: Set up autosomal and X-linked crosses	3 days after lab: pick (2) wild type worms from each cross to new plates (3 plates total); Examine the phenotypes of the progeny – hermaphrodites and males	Homework:Familiarize yourself with the information in the BISC_219/F10:Resources section; Read all of the background information on C. elegans found in BISC_219/F10:_Worm_Info and read the first Series 1: BISC_219/F10:_Gene_Linkage & BISC_219/F10:_Lab_2; Complete Graded Assignment 1 explained at BISC_219/F10: Assignment_1_Lab1
2	Sept. 14 to Sept 20	Complete Series 1: Count and examine phenotypes of autosomal vs. X-linked crosses; Start Series 2: Perform mutant hunt: Pick (3) putative Dpy mutants to separate plates	Examine mutants from hunt: check phenotype – if Dpy then cross L4 mutant hermaphrodites by L4 N2 males (2 plates – duplicate)	Homework:Data Analysis (Results)of your autosomal vs. X-linked testing due next lab. Grading rubric & Assignment info at: BISC_219/F10: Assignment Help- Data Analysis 1
3	Sept. 21 to Sept. 27	Linkage: Linkage: cross males from last cross to the (5) test strains (5 plates total); PAPER DISCUSSION???	3 days after lab: Linkage: transfer (2) L4 hermaphrodites from each cross to new plates (5 plates total)	Homework:
4	Sept. 28 to Oct. 4	Linkage: examine phenotypes and count to determine linkage; Mapping: pick (5) Uncs from linkage plate to separate plates (5 plates total)	3 days after lab: Mapping: Pick (3) double mutants to separate plates (3 plates total); Complementation: Cross Dpy mutant worms to N2 males (2 plates total)	Homework: Start working on Mapping Partial Paper (Introduction section). See BISC_219/F10:Resources and BISC_219/F10: Assignment Help- Classical Genetics: Mapping for help.
5	Oct. 5 to Oct. 12	Mapping: cross N2 males with double mutants (2 plates total); Complementation: pick males from complementation cross #1 and mate with known Dpy strains (5 plates total); RNAi: PCR of gene of interest	3 days after lab: Mapping: pick (2) heterozygotes from previous cross to each of 2 plates (4 plates total); Complementation: examine cross plates for Dpy males	Homework:
6	Oct. 13 to Oct. 19	Mapping: SCORE ; RNAi:Restriction enzyme digest/purfication/ligation/transformation	Determine map distance between your mutant gene and the known reference mutation. Check transformations – notify instructor if no colonies	Homework: Gene Mapping scientific paper due week of 10/29
7	Oct. 20 to Oct. 26	RNAi: Colony PCR to look for bacterial transformants	Day before lab: grow overnight culture of positive colony	Homework:
8	Oct. 28 to Nov. 3	RNAi: Plasmid purification and transformation	Day before lab: grow overnight culture of single colony	Homework:
9	Nov.4 to Nov. 10	RNAi : Induction of bacteria for RNAi feeding; PAPER DISCUSSION??	4 days before lab: Add worms to RNAi media_	Homework:
10	Nov. 11 to Nov. 17	RNAi: SCORING (collection) & RNA purification	_	Homework:
NO LAB	Nov. 18 to Nov. 26	Thanksgiving Break	_	Homework:
11	Nov. 29 to Dec. 3	Series 3: RT PCR Reactions	__	Homework: Work on RNAi Paper – Title, Abstract, Intro, Materials and Methods, Results, Discussion, References – BISC_219/F10: Assignment Help- RNAi scientific paper
12	Dec. 6 to Dec. 10	TBA	TBA	TBA