BISC 219/F10:Calendars/Planner: Difference between revisions
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|'''3 days after lab''':Examine mutants from hunt: check phenotype <BR>– if Dpy then cross L4 mutant hermaphrodites by L4 N2 males<BR> (2 plates – duplicate) | |'''3 days after lab''':Examine mutants from hunt: check phenotype <BR>– if Dpy then cross L4 mutant hermaphrodites by L4 N2 males<BR> (2 plates – duplicate) | ||
| '''Homework:''' Read ALL of Series 2 work performed in Labs 2-6 on our Classical (Forward) Genetics project and write a summary of our experimental question & goals. Outline the experimental process, including all parts of the experimental design: Mutant Hunt, Linkage Analysis, Mapping the Mutation to a chromosome and to a particular location, Complementation Analysis, Determining whether or not the mutation has been previously characterized, & Defining the gene sequence and protein change through DNA sequence analysis. Use this outline to write a 1-2 page summary of how you will determine the exact location and extent of the gene and protein change and include the significance of the mutation in worms and, if possible, broader significance in other species. Graded Assignment 2 explained at [[BISC_219/F10: Assignment Series2_Outline_Summary]]<BR> | | '''Homework:''' Read ALL of Series 2 work performed in Labs 2-6 on our Classical (Forward) Genetics project and write a summary of our experimental question & goals. Outline the experimental process, including all parts of the experimental design: Mutant Hunt, Linkage Analysis, Mapping the Mutation to a chromosome and to a particular location, Complementation Analysis, Determining whether or not the mutation has been previously characterized, & Defining the gene sequence and protein change through DNA sequence analysis. Use this outline to write a 1-2 page summary of how you will determine the exact location and extent of the gene and protein change and include the significance of the mutation in worms and, if possible, broader significance in other species. Graded Assignment 2 explained at [[BISC_219/F10: Assignment Series2_Outline_Summary]]<BR> | ||
Start Data Analysis (Results) | Start Data Analysis (Results)of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. <BR>Grading rubric & Assignment info at:<BR> [[BISC_219/F10: Assignment Help- Data Analysis 1]] | ||
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Revision as of 12:11, 15 August 2010
BISC219 F10 Lab Calendar
| Monday | Tuesday | Wednesday | Thursday | Friday | |
|---|---|---|---|---|---|
| Sept. 6 Labor Day Holiday |
Sept. 7 Lab 1 |
Sept. 8 Lab 1 |
Sept. 9 Lab 1 |
Sept. 10 Lab 1 |
|
| Sept. 13 Lab 1 |
Sept. 14 Lab 2 |
Sept. 15 Lab 2 |
Sept. 16 Lab 2 |
Sept. 17 Lab 2 |
|
| Sept. 20 Lab 2 |
Sept. 21 Lab 3 |
Sept. 22 Lab 3 |
Sept. 23 Lab 3 |
Sept. 24 Lab 3 |
|
| Sept. 27 Lab 3 |
Sept. 28 Lab 4 |
Sept. 29 Lab 4 |
Sept. 30 Lab 4 |
Oct. 1 Lab 4 |
|
| Oct. 4 Lab 4 |
Oct. 5 Lab 5 |
Oct. 6 Lab 5 |
Oct. 7 Lab 5 |
Oct. 8 Lab 5 |
|
| Oct. 11 Fall Break No Lab |
Oct. 12 MON. Sch. Lab 5 |
Oct. 13 Lab 6 |
Oct. 14 Lab 6 |
Oct. 15 Lab 6 |
|
| Oct. 18 Lab 6 |
Oct. 19 Lab 6 |
Oct. 20 Lab 7 |
Oct. 21 Lab 7 |
Oct. 22 Lab 7 |
|
| Oct. 25 Lab 7 |
Oct. 26 Lab 7 |
Oct. 27 Tanner Conf. No Lab |
Oct. 28 Lab 8 |
Oct. 29 Lab 8 |
|
| Nov. 1 Lab 8 |
Nov. 2 Lab 8 |
Nov. 3 Lab 8 |
Nov. 4 Lab 9 |
Nov. 5 Lab 9 |
|
| Nov. 8 Lab 9 |
Nov. 9 Lab 9 |
Nov. 10 Lab 9 |
Nov. 11 Lab 10 |
Nov. 12 Lab 10 |
|
| Nov. 15 Lab 10 |
Nov. 16 Lab 10 |
Nov. 17 Lab 10 |
Nov. 18 NO Lab |
Nov. 19 No Lab |
|
| Nov. 22 No Lab |
Nov. 23 No Lab |
Nov. 24 No Lab |
Nov. 25 Thanksgiving Holiday |
Nov. 26 Thanksgiving Holiday |
|
| Nov. 29 Lab 11 |
Nov. 30 Lab 11 |
Dec. 1 Lab 11 |
Dec. 2 Lab 11 |
Dec. 3 Lab 11 |
|
| Dec. 6 Lab 12 |
Dec. 7 Lab 12 |
Dec. 8 Lab 12 |
Dec. 9 Lab 12 |
Dec. 10 Lab 12 |
Schedule of Experiments
| Series | Title | Lab # |
|---|---|---|
| 1 | Worm Boot Camp; Autosomal and Sex-linked Traits | 1-2 |
| 2 | Mapping a Mutation: Mutant Hunt, Linkage, Mapping, Complementation | 2-6 |
| 3 | RNAi | 5-12 |
BISC219 F10 Weekly Lab Planner
| Lab | Date | In-Lab Work | Outside of Lab Work | Assignment |
|---|---|---|---|---|
| 1 | Sept. 7 to Sept. 13 |
LEARN WORM HUSBANDRY; View worm videos; Make your worm pick; Examine worms: recognize different stages & sexes & differentiate mutations; Practice picking worms; Start Series 1: Set up autosomal and X-linked crosses |
3 days after lab: pick (2) wild type worms from each cross to new plates (3 plates total); Examine the phenotypes of the progeny – hermaphrodites and males |
Homework:Familiarize yourself with the information in the BISC_219/F10:Resources section; Read all of the background information on C. elegans found in BISC_219/F10:_Worm_Info and read the Lab 1 and Lab 2 information about our first Series 1: Autosomal vs. Sex-Linked Inheritance at BISC_219/F10:_Gene_Linkage & BISC_219/F10:_Lab_2; Complete Graded Assignment 1 explained at BISC_219/F10: Assignment_1_Lab1 |
| 2 | Sept. 14 to Sept 20 |
Complete Series 1: Count and examine phenotypes of autosomal vs. X-linked crosses; Start Series 2: Classical (Forward) Genetics- Part 1- Perform mutant hunt: Pick (3) putative Dpy mutants to separate plates |
3 days after lab:Examine mutants from hunt: check phenotype – if Dpy then cross L4 mutant hermaphrodites by L4 N2 males (2 plates – duplicate) |
Homework: Read ALL of Series 2 work performed in Labs 2-6 on our Classical (Forward) Genetics project and write a summary of our experimental question & goals. Outline the experimental process, including all parts of the experimental design: Mutant Hunt, Linkage Analysis, Mapping the Mutation to a chromosome and to a particular location, Complementation Analysis, Determining whether or not the mutation has been previously characterized, & Defining the gene sequence and protein change through DNA sequence analysis. Use this outline to write a 1-2 page summary of how you will determine the exact location and extent of the gene and protein change and include the significance of the mutation in worms and, if possible, broader significance in other species. Graded Assignment 2 explained at BISC_219/F10: Assignment Series2_Outline_Summary Start Data Analysis (Results)of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. |
| 3 | Sept. 21 to Sept. 27 |
Series 2- Part 2: Linkage Analysis: cross males from last cross to the (5) test strains (5 plates total); PAPER DISCUSSION??? |
3 days after lab: Linkage: transfer (2) L4 hermaphrodites from each cross to new plates (5 plates total) |
Homework: Draw crosses and diagram strategy for Series 2 Linkage Analysis due at the beginning of Lab 4. Assignment described at BISC_219/F10: Assignment_Series2_Linkage Testing Crosses
|
| 4 | Sept. 28 to Oct. 4 |
Complete Linkage Analysis: examine phenotypes and count to determine linkage; Part 3: Start Mapping: pick (5) Uncs from linkage plate to separate plates (5 plates total) |
3 days after lab: Mapping: Pick (3) double mutants to separate plates (3 plates total); Part 4: Start Complementation Analysis: Cross Dpy mutant worms to N2 males (2 plates total) |
Homework: Draw crosses and diagram strategy for Mapping the Location of your dpy mutation. Due at the beginning of Lab 5. Assignment described at BISC_219/F10: Assignment_Series2_Mapping Crosses |
| 5 | Oct. 5 to Oct. 12 |
Mapping: cross N2 males with double mutants (2 plates total); Complementation: pick males from complementation cross #1 and mate with known Dpy strains (5 plates total); RNAi: PCR of gene of interest |
3 days after lab: Mapping: pick (2) heterozygotes from previous cross to each of 2 plates (4 plates total); Complementation: examine cross plates for Dpy males |
Homework: Explain complementation analysis in general and specifically how it will be used to confirm and expand your characterization of your dpy mutation. Due at the beginning of Lab 6. Assignment described atBISC_219/F10: Assignment_Series2_Complementation |
| 6 | Oct. 13 to Oct. 19 |
Mapping: SCORE ; RNAi:Restriction enzyme digest/purfication/ligation/transformation |
Determine map distance between your mutant gene and the known reference mutation. Check transformations – notify instructor if no colonies |
Homework: DNA sequencing Analysis due at the beginning of Lab 7. Assignment description at BISC_219/F10: Assignment_Series2_DNA Sequencing Scientific research report on Series2 Classical (Forward) Genetics Project due week of 11/1 at the beginning of lab. 5%/day late penalty! See assignment directions at: BISC 219/F10: Assignment_ Series2_Classical Genetics Paper |
| 7 | Oct. 20 to Oct. 26 |
RNAi: Colony PCR to look for bacterial transformants | Day before lab: grow overnight culture of positive colony | Homework: Continue working on your Series 2 paper |
| 8 | Oct. 28 to Nov. 3 |
RNAi: Plasmid purification and transformation | Day before lab: grow overnight culture of single colony | Homework: Write all Series3 Protocols as Materials & Methods. Assignment described at BISC_219/F10: Assignment_Series3_ Materials and Methods |
| 9 | Nov.4 to Nov. 10 |
RNAi : Induction of bacteria for RNAi feeding; PAPER DISCUSSION?? |
4 days before lab: Add worms to RNAi media_ | Homework: |
| 10 | Nov. 11 to Nov. 17 |
RNAi: SCORING (collection) & RNA purification | _ | Homework: |
| NO LAB | Nov. 18 to Nov. 26 |
Thanksgiving Break | _ | Homework: |
| 11 | Nov. 29 to Dec. 3 |
Series 3: RT PCR Reactions | __ | Homework: Full Scientific Research Report on Reverse Genetics Project – Title, Abstract, Intro, Materials and Methods, Results, Discussion, References See BISC_219/F10:Resources section Guide to Scientific Wriing and BISC_219/F10: Assignment_Series3_Reverse Genetics Paper using RNAi |
| 12 | Dec. 6 to Dec. 10 |
TBA | TBA | Scientific Research Report on Reverse Genetics project DUE Dec. 10 (last day of classes) by 4pm for ALL students Assignment information at BISC_219/F10: Assignment_Series3_Reverse Genetics Paper using RNAi |
