BISC 219/F10:Calendars/Planner
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BISC219 F10 Lab Calendar
| Monday | Tuesday | Wednesday | Thursday | Friday | |
|---|---|---|---|---|---|
| Sept. 6 Labor Day Holiday |
Sept. 7 Lab 1 |
Sept. 8 Lab 1 |
Sept. 9 Lab 1 |
Sept. 10 Lab 1 |
|
| Sept. 13 Lab 1 |
Sept. 14 Lab 2 |
Sept. 15 Lab 2 |
Sept. 16 Lab 2 |
Sept. 17 Lab 2 |
|
| Sept. 20 Lab 2 |
Sept. 21 Lab 3 |
Sept. 22 Lab 3 |
Sept. 23 Lab 3 |
Sept. 24 Lab 3 |
|
| Sept. 27 Lab 3 |
Sept. 28 Lab 4 |
Sept. 29 Lab 4 |
Sept. 30 Lab 4 |
Oct. 1 Lab 4 |
|
| Oct. 4 Lab 4 |
Oct. 5 Lab 5 |
Oct. 6 Lab 5 |
Oct. 7 Lab 5 |
Oct. 8 Lab 5 |
|
| Oct. 11 Fall Break No Lab |
Oct. 12 MON. Sch. Lab 5 |
Oct. 13 Lab 6 |
Oct. 14 Lab 6 |
Oct. 15 Lab 6 |
|
| Oct. 18 Lab 6 |
Oct. 19 Lab 6 |
Oct. 20 Lab 7 |
Oct. 21 Lab 7 |
Oct. 22 Lab 7 |
|
| Oct. 25 Lab 7 |
Oct. 26 Lab 7 |
Oct. 27 Tanner Conf. No Lab |
Oct. 28 Lab 8 |
Oct. 29 Lab 8 |
|
| Nov. 1 Lab 8 |
Nov. 2 Lab 8 |
Nov. 3 Lab 8 |
Nov. 4 Lab 9 |
Nov. 5 Lab 9 |
|
| Nov. 8 Lab 9 |
Nov. 9 Lab 9 |
Nov. 10 Lab 9 |
Nov. 11 Lab 10 |
Nov. 12 Lab 10 |
|
| Nov. 15 Lab 10 |
Nov. 16 Lab 10 |
Nov. 17 Lab 10 |
Nov. 18 NO Lab |
Nov. 19 No Lab |
|
| Nov. 22 No Lab |
Nov. 23 No Lab |
Nov. 24 No Lab |
Nov. 25 Thanksgiving Holiday |
Nov. 26 Thanksgiving Holiday |
|
| Nov. 29 Lab 11 |
Nov. 30 Lab 11 |
Dec. 1 Lab 11 |
Dec. 2 Lab 11 |
Dec. 3 Lab 11 |
|
| Dec. 6 Lab 12 |
Dec. 7 Lab 12 |
Dec. 8 Lab 12 |
Dec. 9 Lab 12 |
Dec. 10 Lab 12 |
Schedule of Experiments
| Series | Title | Lab # |
|---|---|---|
| 1 | Worm Boot Camp; Autosomal and Sex-linked Traits | 1-2 |
| 2 | Mapping a Mutation: Mutant Hunt, Linkage, Mapping, Complementation | 2-6 |
| 3 | RNAi | 5-12 |
Graded Assignments
| Lab Assigned | Lab Due | Assignment description |
Point Value (Total 200) |
|---|---|---|---|
| Lab 1 | Lab 2 | Complete entry survey (2.5pts); Submit preliminary cross results Day 3 (1 pt); Summarize experimental question, design; Diagram crosses; Explain data interpretation (9 pts) BISC_219/F10: Assignment_1_Lab1 BISC_219/F10: Assignment_Series2_Outline_Summary |
12.5 |
| Lab 2 | Lab 3 | Statement of Series 2 experimental question & goals. Outline of experimental process including : Mutant Hunt, Linkage Analysis, Mapping the Mutation to a chromosome and to a particular location, Complementation Analysis, Determining whether or not the mutation has been previously characterized, & Defining the gene sequence and protein change through DNA sequence analysis. 1-2 page summary of how you will determine the exact location of the gene and the sequence change likely to be associated with a phenotypic anomoly.BISC_219/F10: Assignment_Series2_Outline_Summary | 10 |
| Lab 2 | Lab 4 | Results Section BISC 219/F10: Assignment Help- Data Analysis 1 Autosomal vs. Sex linked inheritance |
30 |
| Lab 3 | Lab 4 | ||
| Lab 4 | Lab 5 | ||
| Lab 5 | Lab 6 | ||
| Lab 6 | Lab 7 | ||
| Lab 7 | Lab 8 | ||
| Lab 8 | Lab 9 | ||
| Lab 9 | Lab 10 | ||
| Lab 10 | Lab 11 | ||
| Lab 11 | Lab 12 | ||
| Other | Discretionary Points: Preparation & Participation |
5 |
BISC219 F10 Weekly Lab Planner
| Lab | Date | In-Lab Work | Outside of Lab Work | Assignment |
|---|---|---|---|---|
| 1 | Sept. 7 to Sept. 13 |
LEARN WORM HUSBANDRY; View worm videos; Make your worm pick; Examine worms: recognize different stages & sexes & differentiate mutations; Practice picking worms; Start Series 1: Set up autosomal and X-linked crosses |
3 days after lab: pick (2) wild type worms from each cross to new plates (3 plates total); Examine the phenotypes of the progeny – hermaphrodites and males |
Homework:Familiarize yourself with the information in the BISC_219/F10:Resources section; Read all of the background information on C. elegans found in BISC_219/F10:_Worm_Info and read the first Series 1: BISC_219/F10:_Gene_Linkage & BISC_219/F10:_Lab_2; Complete Graded Assignment 1 explained at BISC_219/F10: Assignment_1_Lab1 |
| 2 | Sept. 14 to Sept 20 |
Complete Series 1: Count and examine phenotypes of autosomal vs. X-linked crosses; Start Series 2: Mapping a Mutation- Part 1- Perform mutant hunt: Pick (3) putative Dpy mutants to separate plates |
3 days after lab:Examine mutants from hunt: check phenotype – if Dpy then cross L4 mutant hermaphrodites by L4 N2 males (2 plates – duplicate) |
Homework: Read ALL of Series 2 performed in Labs 2-6 on Mapping a Mutation and write a summary of our experimental question & goals. Outline the experimental process of mapping the mutation (include all parts of the experimental design: Mutant Hunt, Linkage Analysis, Mapping the Mutation to a chromosome and to a particular location, Complementation Analysis, Determining whether or not the mutation has been previously characterized, & Defining the gene sequence and protein change through DNA sequence analysis). Use this outline to write a 1-2 page summary of how you will determine the exact location of the gene and the sequence change likely to be associated with a phenotypic anomoly. Graded Assignment 2 explained at BISC_219/F10: Assignment Series2_Outline_Summary |
| 3 | Sept. 21 to Sept. 27 |
Series 2- Part 2: Linkage: cross males from last cross to the (5) test strains (5 plates total); PAPER DISCUSSION??? |
3 days after lab: Linkage: transfer (2) L4 hermaphrodites from each cross to new plates (5 plates total) |
Homework: Data Analysis (Results)of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. Grading rubric & Assignment info at: BISC_219/F10: Assignment Help- Data Analysis 1 |
| 4 | Sept. 28 to Oct. 4 |
Complete Linkage Analysis: examine phenotypes and count to determine linkage; Part 3: Start Mapping: pick (5) Uncs from linkage plate to separate plates (5 plates total) |
3 days after lab: Mapping: Pick (3) double mutants to separate plates (3 plates total); Part 4: Start Complementation Analysis: Cross Dpy mutant worms to N2 males (2 plates total) |
Homework: Series II Partial Paper on Mapping a Mutation: Write Introduction section. See BISC_219/F10:Resources and BISC_219/F10: Assignment - Partial Paper Mapping a Mutation Intro Section for help. |
| 5 | Oct. 5 to Oct. 12 |
Mapping: cross N2 males with double mutants (2 plates total); Complementation: pick males from complementation cross #1 and mate with known Dpy strains (5 plates total); RNAi: PCR of gene of interest |
3 days after lab: Mapping: pick (2) heterozygotes from previous cross to each of 2 plates (4 plates total); Complementation: examine cross plates for Dpy males |
Homework: |
| 6 | Oct. 13 to Oct. 19 |
Mapping: SCORE ; RNAi:Restriction enzyme digest/purfication/ligation/transformation |
Determine map distance between your mutant gene and the known reference mutation. Check transformations – notify instructor if no colonies |
Homework: Mapping a Mutation partial scientific paper due week of 10/29 |
| 7 | Oct. 20 to Oct. 26 |
RNAi: Colony PCR to look for bacterial transformants | Day before lab: grow overnight culture of positive colony | Homework: |
| 8 | Oct. 28 to Nov. 3 |
RNAi: Plasmid purification and transformation | Day before lab: grow overnight culture of single colony | Homework: |
| 9 | Nov.4 to Nov. 10 |
RNAi : Induction of bacteria for RNAi feeding; PAPER DISCUSSION?? |
4 days before lab: Add worms to RNAi media_ | Homework: |
| 10 | Nov. 11 to Nov. 17 |
RNAi: SCORING (collection) & RNA purification | _ | Homework: |
| NO LAB | Nov. 18 to Nov. 26 |
Thanksgiving Break | _ | Homework: |
| 11 | Nov. 29 to Dec. 3 |
Series 3: RT PCR Reactions | __ | Homework: Full RNAi Scientific Paper – Title, Abstract, Intro, Materials and Methods, Results, Discussion, References – BISC_219/F10: Assignment- RNAi scientific paper |
| 12 | Dec. 6 to Dec. 10 |
TBA | TBA | TBA |
