BISC 219/F10:Gene Mapping: Difference between revisions
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| Determine map distance between your Dpy and Unc marker<br> | | Determine map distance between your Dpy and Unc marker<br> | ||
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==Links to Labs& Project Info== | |||
Series1:<BR> | |||
[[BISC 219/F10: Worm Info| Worm Info]] <br> | |||
[[BISC 219/F10: Gene Linkage| Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start]]<BR> | |||
[[BISC 219/F10: Lab 2 | Lab 2: Sex-Linked or Autosomal Finale]]<br> | |||
Series2:<BR> | |||
[[BISC 219/F10: Gene Mapping Info | Background: Classical Forward Genetics and Gene Mapping]]<br> | |||
[[BISC 219/F10: Lab 2 Mutant Hunt | Lab 2: Mutant Hunt]]<br> | |||
[[BISC 219/F10: Lab 3 | Lab 3: Linkage Test Part 1]]<br> | |||
[[BISC 219/F10: Lab 4 | Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br> | |||
[[BISC 219/F10: Lab 5 | Lab 5: Mapping Con't]]<br> | |||
[[BISC 219/F10: Lab 6 | Lab 6: Finish Complementation; Mapping Con't]]<BR> | |||
[[BISC 219/F10: Lab 7 | Lab 7: Complete Mapping: Score]]<br> | |||
Series3:<BR> | |||
[[BISC 219/F10:RNA interference | Schedule of Reverse Genetics Project]]<BR> | |||
[[BISC 219/F10:RNAi General Information| RNAi General Information]] <br> | |||
[[BISC 219/F10:Media Recipes | Media Recipes]]<br> | |||
[[BISC 219/F10: RNAi Lab 5 | Lab 5: Picking your gene to RNAi]]<br> | |||
[[BISC 219/F10: RNAi Lab 6 | Lab 6: Cloning your gene of interest]]<br> | |||
[[BISC 219/F10: RNAi Lab 7 | Lab 7: Picking your transformant]]<br> | |||
[[BISC 219/F10: RNAi Lab 8 | Lab 8: Plasmid purification and transformation]]<br> | |||
[[BISC 219/F10: RNAi Lab 9 | Lab 9: Induction of bacteria for RNAi]]<br> | |||
[[BISC 219/F10: RNAi Lab 10 | Lab 10: Scoring your worms and RNA purification]]<br> | |||
[[BISC 219/F10: RNAi Lab 11 | Lab 11: RT PCR reactions]]<br><br> |
Revision as of 17:33, 30 September 2010
Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping con't; complete complementation
Lab 6: DNA sequencing analysis; Mapping con't
Lab 7: Complete Mapping: Score!
Schedule of Experiments for Series 2
Links to Labs& Project Info
Series1:
Worm Info
Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start
Lab 2: Sex-Linked or Autosomal Finale
Series2:
Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping Con't
Lab 6: Finish Complementation; Mapping Con't
Lab 7: Complete Mapping: Score
Series3:
Schedule of Reverse Genetics Project
RNAi General Information
Media Recipes
Lab 5: Picking your gene to RNAi
Lab 6: Cloning your gene of interest
Lab 7: Picking your transformant
Lab 8: Plasmid purification and transformation
Lab 9: Induction of bacteria for RNAi
Lab 10: Scoring your worms and RNA purification
Lab 11: RT PCR reactions
Lab # | Dates | Activity | Outside Activity |
---|---|---|---|
2 | 9/14 - 9/20 | Mutant Hunt | Confirm mutant phenotype and set up first cross |
3 | 9/21 - 9/27 | Linkage crosses - heterozygous males with Unc hermaphrodites | Pick hermaphrodites for selfing |
4 | 9/28 - 10/4 | Examine phenotypes to determine linkage Mapping: Pick Unc non-Dpy to separate plates |
Mapping: Pick putative double mutants Complementation: cross unknown Dpy worms to wild type males |
5 | 10/5 - 10/12 | Mapping: cross double mutants with wild type males Complementation: cross heterozygous hermaphrodites with known Dpy mutants |
Mapping: Maintain Double mutant pick heterozygotes to individual plates Complementation: examine crosses for Dpy MALES |
6 | 10/13 - 10/19 | DNA Sequencing Analysis Examine sequencing data to determine the lesion in your gene of interest & significance in gene product |
Bioinformatics: Use Wormbase or other BLAST to learn more about the gene in other species |
7 | 10/20 - 10/26 | Complete Mapping SCORE! Find recombinant frequency |
Determine map distance between your Dpy and Unc marker |