BISC 219/F10:Gene Mapping: Difference between revisions

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| Determine map distance between your Dpy and Unc marker<br>
| Determine map distance between your Dpy and Unc marker<br>
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==Links to Labs& Project Info==
Series1:<BR>
[[BISC 219/F10: Worm Info| Worm Info]] <br>
[[BISC 219/F10: Gene Linkage| Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start]]<BR>
[[BISC 219/F10: Lab 2  | Lab 2: Sex-Linked or Autosomal Finale]]<br>
Series2:<BR>
[[BISC 219/F10: Gene Mapping Info | Background: Classical Forward Genetics and Gene Mapping]]<br>
[[BISC 219/F10: Lab 2 Mutant Hunt | Lab 2: Mutant Hunt]]<br>
[[BISC 219/F10: Lab 3  | Lab 3: Linkage Test Part 1]]<br>
[[BISC 219/F10: Lab 4  | Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>
[[BISC 219/F10: Lab 5  | Lab 5: Mapping Con't]]<br>
[[BISC 219/F10: Lab 6 | Lab 6: Finish Complementation; Mapping Con't]]<BR>
[[BISC 219/F10: Lab 7  | Lab 7: Complete Mapping: Score]]<br>
Series3:<BR>
[[BISC 219/F10:RNA interference | Schedule of Reverse Genetics Project]]<BR>
[[BISC 219/F10:RNAi General Information| RNAi General Information]] <br>
[[BISC 219/F10:Media Recipes | Media Recipes]]<br>
[[BISC 219/F10: RNAi Lab 5  | Lab 5: Picking your gene to RNAi]]<br>
[[BISC 219/F10: RNAi Lab 6  | Lab 6: Cloning your gene of interest]]<br>
[[BISC 219/F10: RNAi Lab 7  | Lab 7: Picking your transformant]]<br>
[[BISC 219/F10: RNAi Lab 8  | Lab 8: Plasmid purification and transformation]]<br>
[[BISC 219/F10: RNAi Lab 9  | Lab 9: Induction of bacteria for RNAi]]<br>
[[BISC 219/F10: RNAi Lab 10 | Lab 10: Scoring your worms and RNA purification]]<br>
[[BISC 219/F10: RNAi Lab 11 | Lab 11: RT PCR reactions]]<br><br>

Revision as of 18:25, 30 September 2010

Wellesley College BISC 219 Genetics

Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping con't; complete complementation
Lab 6: DNA sequencing analysis; Mapping con't
Lab 7: Complete Mapping: Score!

Schedule of Experiments for Series 2

Lab # Dates Activity Outside Activity
2 9/14 - 9/20 Mutant Hunt Confirm mutant phenotype and set up first cross
3 9/21 - 9/27 Linkage crosses - heterozygous males with Unc hermaphrodites Pick hermaphrodites for selfing
4 9/28 - 10/4 Examine phenotypes to determine linkage

Mapping: Pick Unc non-Dpy to separate plates

Mapping: Pick putative double mutants

Complementation: cross unknown Dpy worms to wild type males

5 10/5 - 10/12 Mapping: cross double mutants with wild type males

Complementation: cross heterozygous hermaphrodites with known Dpy mutants

Mapping: Maintain Double mutant pick heterozygotes to individual plates

Complementation: examine crosses for Dpy MALES

6 10/13 - 10/19 DNA Sequencing Analysis

Examine sequencing data to determine the lesion in your gene of interest & significance in gene product

Bioinformatics: Use Wormbase or other BLAST to learn more about the gene in other species
7 10/20 - 10/26 Complete Mapping

SCORE! Find recombinant frequency

Determine map distance between your Dpy and Unc marker
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