BISC 219/F10:Gene Mapping: Difference between revisions

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Line 28: Line 28:
| 9/28 - 10/4  
| 9/28 - 10/4  
| Examine phenotypes to determine linkage<br>
| Examine phenotypes to determine linkage<br>
'''Mapping:''' Pick Unc non-Dpy to separate plates
'''Mapping:''' Pick Unc non-Dpy to separate plates;'''Complementation:''' cross unknown Dpy worms to wild type males
| '''Mapping:''' Pick putative double mutants<br>
| '''Mapping:''' Pick putative double mutants<br>'''Complementation:''' cross heterozygous hermaphrodites with known Dpy mutants
'''Complementation:''' cross unknown Dpy worms to wild type males
 
|-
|-
! 5
! 5
| 10/5 - 10/12
| 10/5 - 10/12
| '''Mapping:''' cross double mutants with wild type males<br>
| '''Mapping:''' Maintain Double mutant pick heterozygotes to individual plates<br>'''Complementation:''' examine crosses for Dpy '''MALES'''
'''Complementation:''' cross heterozygous hermaphrodites with known Dpy mutants
| Nothing!
| '''Mapping:'''  Maintain Double mutant pick heterozygotes to individual plates<br>
 
'''Complementation:''' examine crosses for Dpy '''MALES'''
|-
|-
! 6
! 6
| 10/13 - 10/19
| 10/13 - 10/19
| ''' DNA Sequencing Analysis'''<br>
| ''' DNA Sequencing Analysis''';<br>Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)
Examine sequencing data to determine the lesion in your gene of interest & significance in gene product
Examine sequencing data to determine the lesion in your gene of interest & significance in gene product; Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)
| Bioinformatics: Use Wormbase or other BLAST to learn more about the gene in other species<br>
| Bioinformatics: Use Wormbase and BLAST to learn more about the gene in other species<br>
|-
|-
! 7
! 7

Revision as of 18:30, 30 September 2010

Wellesley College BISC 219 Genetics

Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping con't; complete complementation
Lab 6: DNA sequencing analysis; Mapping con't
Lab 7: Complete Mapping: Score!

Schedule of Experiments for Series 2

Lab # Dates Activity Outside Activity
2 9/14 - 9/20 Mutant Hunt Confirm mutant phenotype and set up first cross
3 9/21 - 9/27 Linkage crosses - heterozygous males with Unc hermaphrodites Pick hermaphrodites for selfing
4 9/28 - 10/4 Examine phenotypes to determine linkage

Mapping: Pick Unc non-Dpy to separate plates;Complementation: cross unknown Dpy worms to wild type males

Mapping: Pick putative double mutants
Complementation: cross heterozygous hermaphrodites with known Dpy mutants
5 10/5 - 10/12 Mapping: Maintain Double mutant pick heterozygotes to individual plates
Complementation: examine crosses for Dpy MALES 		Nothing!
6 10/13 - 10/19 DNA Sequencing Analysis;
Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)

Examine sequencing data to determine the lesion in your gene of interest & significance in gene product; Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)

Bioinformatics: Use Wormbase and BLAST to learn more about the gene in other species
7 10/20 - 10/26 Complete Mapping

SCORE! Find recombinant frequency

Determine map distance between your Dpy and Unc marker
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