BISC 219/F10:Gene Mapping: Difference between revisions
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[[BISC 219/F10: Lab 3 | Lab 3: Linkage Test Part 1]]<br> | [[BISC 219/F10: Lab 3 | Lab 3: Linkage Test Part 1]]<br> | ||
[[BISC 219/F10: Lab 4 | Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br> | [[BISC 219/F10: Lab 4 | Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br> | ||
[[BISC 219/F10: Lab 5 | Lab 5: Mapping | [[BISC 219/F10: Lab 5 | Lab 5: Mapping con't; complete complementation]]<br> | ||
[[BISC 219/F10: Lab 6 | Lab 6: | [[BISC 219/F10: Lab 6 | Lab 6: DNA sequencing analysis; Mapping con't ]]<br> | ||
[[BISC 219/F10: Lab 7 | Lab 7: Complete Mapping: Score!]] | |||
=='''Schedule of Experiments'''== | =='''Schedule of Experiments for Series 2'''== | ||
{| border="1" | {| border="1" | ||
|+ | |+ | ||
| Line 27: | Line 28: | ||
| 9/28 - 10/4 | | 9/28 - 10/4 | ||
| Examine phenotypes to determine linkage<br> | | Examine phenotypes to determine linkage<br> | ||
'''Mapping:''' Pick | '''Mapping:''' Pick Unc non-Dpy to separate plates;<BR>'''Complementation:''' cross unknown Dpy worms to wild type males | ||
| '''Mapping:''' Pick putative double mutants<br> | | '''Mapping:''' Pick putative double mutants<br>'''Complementation:''' cross heterozygous males with known Dpy mutants | ||
'''Complementation:''' cross | |||
|- | |- | ||
! 5 | ! 5 | ||
| 10/5 - 10/12 | | 10/5 - 10/12 | ||
| '''Mapping:''' | | '''Mapping:''' Maintain double mutants (d u/d u): pick a few to individual plates<br>'''Complementation:''' examine crosses for Dpy '''MALES''' | ||
| Nothing! | |||
'''Complementation:''' examine crosses for Dpy '''MALES''' | |||
|- | |- | ||
! 6 | ! 6 | ||
| 10/13 - 10/19 | | 10/13 - 10/19 | ||
| ''' | | ''' DNA Sequencing Analysis''':Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;<br>'''Mapping''':Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du) | ||
Examine sequencing data to determine the lesion in your gene of interest | | '''Bioinformatics''':Use Wormbase and BLAST to learn more about the gene in other species;<br>'''Mapping:'''Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u) | ||
|- | |||
! 7 | |||
| 10/20 - 10/26 | |||
| ''' Complete Mapping'''<br> | |||
SCORE! Find recombinant frequency | |||
| Determine map distance between your Dpy and Unc marker<br> | | Determine map distance between your Dpy and Unc marker<br> | ||
|- | |- | ||
Latest revision as of 18:36, 30 September 2010
Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping con't; complete complementation
Lab 6: DNA sequencing analysis; Mapping con't
Lab 7: Complete Mapping: Score!
Schedule of Experiments for Series 2
| Lab # | Dates | Activity | Outside Activity |
|---|---|---|---|
| 2 | 9/14 - 9/20 | Mutant Hunt | Confirm mutant phenotype and set up first cross |
| 3 | 9/21 - 9/27 | Linkage crosses - heterozygous males with Unc hermaphrodites | Pick hermaphrodites for selfing |
| 4 | 9/28 - 10/4 | Examine phenotypes to determine linkage Mapping: Pick Unc non-Dpy to separate plates; |
Mapping: Pick putative double mutants Complementation: cross heterozygous males with known Dpy mutants |
| 5 | 10/5 - 10/12 | Mapping: Maintain double mutants (d u/d u): pick a few to individual plates Complementation: examine crosses for Dpy MALES |
Nothing! |
| 6 | 10/13 - 10/19 | DNA Sequencing Analysis:Examine sequencing data to determine the lesion in your gene of interest & significance in gene product; Mapping:Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du) |
Bioinformatics:Use Wormbase and BLAST to learn more about the gene in other species; Mapping:Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u) |
| 7 | 10/20 - 10/26 | Complete Mapping SCORE! Find recombinant frequency |
Determine map distance between your Dpy and Unc marker |
