BISC 219/F10:Gene Mapping
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Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping Part 2
Lab 6: Score & DNA sequencing analysis
Schedule of Experiments for Series 2
| Lab # | Dates | Activity | Outside Activity |
|---|---|---|---|
| 2 | 9/14 - 9/20 | Mutant Hunt | Confirm mutant phenotype and set up first cross |
| 3 | 9/21 - 9/27 | Linkage crosses - heterozygous males with Unc hermaphrodites | Pick hermaphrodites for selfing |
| 4 | 9/28 - 10/4 | Examine phenotypes to determine linkage Mapping: Pick Dpy non-Uncs to separate plates |
Mapping: Pick putative double mutants Complementation: cross unknown Dpy worms to wild type males |
| 5 | 10/5 - 10/12 | Mapping: cross double mutants with wild type males Complementation: cross heterozygous hermaphrodites with known Dpy mutants |
Mapping: pick heterozygotes to individual plates Complementation: examine crosses for Dpy MALES |
| 6 | 10/13 - 10/19 | Score! & DNA Sequencing Analysis Examine sequencing data to determine the lesion in your gene of interest |
Determine map distance between your Dpy and Unc marker Work on paper |
