BISC 219/F10:Media Recipes

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(New page: {{Template:BISC 219/F10}} <div style="padding: 10px; width: 720px; border: 5px solid #2171B7;"> == Plasmid Isolation and Transformation Solutions == {| border="1" |+ ! Solution !! Compos...)
(Plasmid Isolation and Transformation Solutions)
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! Solution !! Composition   
! Solution !! Composition   
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! Solution 1   
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! Solution 1*  
| 50 mM glucose<br> 10 mM EDTA <br> 25 mM Tris, pH 8   
| 50 mM glucose<br> 10 mM EDTA <br> 25 mM Tris, pH 8   
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|-
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! Solution 2
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! Solution 2*
| 0.2 M NaOH <br> 1% SDS
| 0.2 M NaOH <br> 1% SDS
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|-
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! Solution 3
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! Solution 3*
| 2.7M Potassium Acetate <br> 6.6M Acetic Acid
| 2.7M Potassium Acetate <br> 6.6M Acetic Acid
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| 1% Tryptone <br> 0.5% Yeast Extract <br> 1% NaCl <br> 2% agar in plates
| 1% Tryptone <br> 0.5% Yeast Extract <br> 1% NaCl <br> 2% agar in plates
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|-
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! Ampicillin
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! Ampicillin*
| Stock = 50 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
| Stock = 50 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
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|-
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! Tetracycline
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! Tetracycline*
| Stock = 12.5mg/ml in 50% EtOH <br> use 1:1000 in liquid  <br>  1:1000 in agar
| Stock = 12.5mg/ml in 50% EtOH <br> use 1:1000 in liquid  <br>  1:1000 in agar
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'''*NOTE: These concentrations are STOCK concentrations - not working concentrations!'''

Revision as of 14:23, 10 August 2010

Plasmid Isolation and Transformation Solutions

Solution Composition
Solution 1* 50 mM glucose
10 mM EDTA
25 mM Tris, pH 8
Solution 2* 0.2 M NaOH
1% SDS
Solution 3* 2.7M Potassium Acetate
6.6M Acetic Acid
Luria Broth 1% Tryptone
0.5% Yeast Extract
1% NaCl
2% agar in plates
Ampicillin* Stock = 50 mg/ml in water
use 1:1000 in liquid cultures
1:500 in agar
Tetracycline* Stock = 12.5mg/ml in 50% EtOH
use 1:1000 in liquid
1:1000 in agar
IPTG Stock = 0.5 M
use 0.5 mM in cultures
*NOTE: These concentrations are STOCK concentrations - not working concentrations!
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