BISC 219/F10:Media Recipes

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(Plasmid Isolation and Transformation Solutions)
(Plasmid Isolation and Transformation Solutions)
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'''*NOTE: These concentrations are STOCK concentrations - not working concentrations!'''
'''*NOTE: These concentrations are STOCK concentrations - not working concentrations!'''
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==Links to Labs& Project Info==
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Series1:<BR>
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[[BISC 219/F10: Worm Info| Worm Info]] <br>
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[[BISC 219/F10: Gene Linkage| Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start]]<BR>
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[[BISC 219/F10: Lab 2  | Lab 2: Sex-Linked or Autosomal Finale]]<br>
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Series2:<BR>
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[[BISC 219/F10: Gene Mapping Info | Background: Classical Forward Genetics and Gene Mapping]]<br>
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[[BISC 219/F10: Lab 2 Mutant Hunt | Lab 2: Mutant Hunt]]<br>
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[[BISC 219/F10: Lab 3  | Lab 3: Linkage Test Part 1]]<br>
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[[BISC 219/F10: Lab 4  | Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>
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[[BISC 219/F10: Lab 5  | Lab 5: Mapping Part 2]]<br>
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[[BISC 219/F10: Lab 6  | Lab 6: Score & DNA sequencing analysis]]<br>
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Series3:<BR>
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[[BISC 219/F10:RNA interference | Schedule of Reverse Genetics Project]]<BR>
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[[BISC 219/F10:RNAi General Information| RNAi General Information]] <br>
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[[BISC 219/F10:Media Recipes | Media Recipes]]<br>
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[[BISC 219/F10: RNAi Lab 5  | Lab 5: Picking your gene to RNAi]]<br>
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[[BISC 219/F10: RNAi Lab 6  | Lab 6: Cloning your gene of interest]]<br>
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[[BISC 219/F10: RNAi Lab 7  | Lab 7: Picking your transformant]]<br>
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[[BISC 219/F10: RNAi Lab 8  | Lab 8: Plasmid purification and transformation]]<br>
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[[BISC 219/F10: RNAi Lab 9  | Lab 9: Induction of bacteria for RNAi]]<br>
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[[BISC 219/F10: RNAi Lab 10 | Lab 10: Scoring your worms and RNA purification]]<br>
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[[BISC 219/F10: RNAi Lab 11 | Lab 11: RT PCR reactions]]<br><br>

Revision as of 15:19, 3 September 2010

Plasmid Isolation and Transformation Solutions

Solution Composition
Solution 1* 50 mM glucose
10 mM EDTA
25 mM Tris, pH 8
Solution 2* 0.2 M NaOH
1% SDS
Solution 3* 2.7M Potassium Acetate
6.6M Acetic Acid
Luria Broth 1% Tryptone
0.5% Yeast Extract
1% NaCl
2% agar in plates
Ampicillin* Stock = 50 mg/ml in water
use 1:1000 in liquid cultures
1:500 in agar
Tetracycline* Stock = 12.5mg/ml in 50% EtOH
use 1:1000 in liquid
1:1000 in agar
IPTG Stock = 0.5 M
use 0.5 mM in cultures

*NOTE: These concentrations are STOCK concentrations - not working concentrations!

Links to Labs& Project Info

Series1:
Worm Info
Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start
Lab 2: Sex-Linked or Autosomal Finale
Series2:
Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping Part 2
Lab 6: Score & DNA sequencing analysis
Series3:
Schedule of Reverse Genetics Project
RNAi General Information
Media Recipes
Lab 5: Picking your gene to RNAi
Lab 6: Cloning your gene of interest
Lab 7: Picking your transformant
Lab 8: Plasmid purification and transformation
Lab 9: Induction of bacteria for RNAi
Lab 10: Scoring your worms and RNA purification

Lab 11: RT PCR reactions

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