BISC 219/F10:Media Recipes

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(Links to Labs& Project Info)
(Links to Labs& Project Info)
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[[BISC 219/F10: Lab 3  | Lab 3: Linkage Test Part 1]]<br>
[[BISC 219/F10: Lab 3  | Lab 3: Linkage Test Part 1]]<br>
[[BISC 219/F10: Lab 4  | Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>
[[BISC 219/F10: Lab 4  | Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br>
-
[[BISC 219/F10: Lab 5  | Lab 5: Mapping Con't]]<br>
+
[[BISC 219/F10: Lab 5  | Lab 5: Finish Complementation; Mapping Con't]]<br>
-
[[BISC 219/F10: Lab 6 | Lab 6: Finish Complementation; Mapping Con't]]<BR>
+
[[BISC 219/F10: Lab 6 | Lab 6: DNA sequence analysis; Mapping Con't]]<BR>
[[BISC 219/F10: Lab 7  | Lab 7: Complete Mapping: Score]]<br>
[[BISC 219/F10: Lab 7  | Lab 7: Complete Mapping: Score]]<br>
Series3:<BR>
Series3:<BR>

Revision as of 20:54, 30 September 2010

Plasmid Isolation and Transformation Solutions

Solution Composition
Solution 1* 50 mM glucose
10 mM EDTA
25 mM Tris, pH 8
Solution 2* 0.2 M NaOH
1% SDS
Solution 3* 2.7M Potassium Acetate
6.6M Acetic Acid
Luria Broth 1% Tryptone
0.5% Yeast Extract
1% NaCl
2% agar in plates
Ampicillin* Stock = 50 mg/ml in water
use 1:1000 in liquid cultures
1:500 in agar
Tetracycline* Stock = 12.5mg/ml in 50% EtOH
use 1:1000 in liquid
1:1000 in agar
IPTG Stock = 0.5 M
use 0.5 mM in cultures

*NOTE: These concentrations are STOCK concentrations - not working concentrations!

Links to Labs& Project Info

Series1:
Worm Info
Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start
Lab 2: Sex-Linked or Autosomal Finale
Series2:
Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Finish Complementation; Mapping Con't
Lab 6: DNA sequence analysis; Mapping Con't
Lab 7: Complete Mapping: Score
Series3:
Schedule of Reverse Genetics Project
RNAi General Information
Media Recipes
Lab 5: Picking your gene to RNAi
Lab 6: Cloning your gene of interest
Lab 7: Picking your transformant
Lab 8: Plasmid purification and transformation
Lab 9: Induction of bacteria for RNAi
Lab 10: Scoring your worms and RNA purification

Lab 11: RT PCR reactions

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