BISC 219/F10:Media Recipes

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(Plasmid Isolation and Transformation Solutions)
(Plasmid Isolation and Transformation Solutions)
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! Solution 1*   
! Solution 1*   
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| 50 mM glucose<br> 10 mM EDTA <br> 25 mM Tris, pH 8   
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| 50 mM glucose<br> 10 mM EDTA (Ethylenediaminetetraacetic acid) <br> 25 mM Tris, pH 8   
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! Solution 2*
! Solution 2*

Revision as of 11:16, 15 October 2010

Plasmid Isolation and Transformation Solutions

Solution Composition
Solution 1* 50 mM glucose
10 mM EDTA (Ethylenediaminetetraacetic acid)
25 mM Tris, pH 8
Solution 2* 0.2 M NaOH
1% SDS (sodium dodecyl sulfate)
Solution 3* 2.7M Potassium Acetate
6.6M Acetic Acid
Luria-Bertoni Medium (LB) 1% Tryptone
0.5% Yeast Extract
1% NaCl
2% agar in plates
Ampicillin* Stock = 50 mg/ml in water
use 1:1000 in liquid cultures
1:500 in agar
Tetracycline* Stock = 12.5mg/ml in 50% EtOH
use 1:1000 in liquid
1:1000 in agar
IPTG Stock = 0.5 M
use 0.5 mM in cultures

*NOTE: These concentrations are STOCK concentrations - not working concentrations!

Links to Labs& Project Info

Series1:
Worm Info
Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start
Lab 2: Sex-Linked or Autosomal Finale
Series2:
Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Finish Complementation; Mapping Con't
Lab 6: DNA sequence analysis; Mapping Con't
Lab 7: Complete Mapping: Score
Series3:
Schedule of Reverse Genetics Project
RNAi General Information
Media Recipes
Lab 5: Picking your gene to RNAi
Lab 6: Cloning your gene of interest
Lab 7: Picking your transformant
Lab 8: Plasmid purification and transformation
Lab 9: Induction of bacteria for RNAi
Lab 10: Scoring your worms and RNA purification

Lab 11: RT PCR reactions

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