BISC 219/F10:Media Recipes: Difference between revisions
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'''*NOTE: These concentrations are STOCK concentrations - not working concentrations!''' | '''*NOTE: These concentrations are STOCK concentrations - not working concentrations!''' | ||
==Links to Labs& Project Info== | |||
Series1:<BR> | |||
[[BISC 219/F10: Worm Info| Worm Info]] <br> | |||
[[BISC 219/F10: Gene Linkage| Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start]]<BR> | |||
[[BISC 219/F10: Lab 2 | Lab 2: Sex-Linked or Autosomal Finale]]<br> | |||
Series2:<BR> | |||
[[BISC 219/F10: Gene Mapping Info | Background: Classical Forward Genetics and Gene Mapping]]<br> | |||
[[BISC 219/F10: Lab 2 Mutant Hunt | Lab 2: Mutant Hunt]]<br> | |||
[[BISC 219/F10: Lab 3 | Lab 3: Linkage Test Part 1]]<br> | |||
[[BISC 219/F10: Lab 4 | Lab 4: Linkage Test Part 2, Mapping and Complementation]]<br> | |||
[[BISC 219/F10: Lab 5 | Lab 5: Mapping Part 2]]<br> | |||
[[BISC 219/F10: Lab 6 | Lab 6: Score & DNA sequencing analysis]]<br> | |||
Series3:<BR> | |||
[[BISC 219/F10:RNA interference | Schedule of Reverse Genetics Project]]<BR> | |||
[[BISC 219/F10:RNAi General Information| RNAi General Information]] <br> | |||
[[BISC 219/F10:Media Recipes | Media Recipes]]<br> | |||
[[BISC 219/F10: RNAi Lab 5 | Lab 5: Picking your gene to RNAi]]<br> | |||
[[BISC 219/F10: RNAi Lab 6 | Lab 6: Cloning your gene of interest]]<br> | |||
[[BISC 219/F10: RNAi Lab 7 | Lab 7: Picking your transformant]]<br> | |||
[[BISC 219/F10: RNAi Lab 8 | Lab 8: Plasmid purification and transformation]]<br> | |||
[[BISC 219/F10: RNAi Lab 9 | Lab 9: Induction of bacteria for RNAi]]<br> | |||
[[BISC 219/F10: RNAi Lab 10 | Lab 10: Scoring your worms and RNA purification]]<br> | |||
[[BISC 219/F10: RNAi Lab 11 | Lab 11: RT PCR reactions]]<br><br> |
Revision as of 12:19, 3 September 2010
Plasmid Isolation and Transformation Solutions
Solution | Composition |
---|---|
Solution 1* | 50 mM glucose 10 mM EDTA 25 mM Tris, pH 8 |
Solution 2* | 0.2 M NaOH 1% SDS |
Solution 3* | 2.7M Potassium Acetate 6.6M Acetic Acid |
Luria Broth | 1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar in plates |
Ampicillin* | Stock = 50 mg/ml in water use 1:1000 in liquid cultures 1:500 in agar |
Tetracycline* | Stock = 12.5mg/ml in 50% EtOH use 1:1000 in liquid 1:1000 in agar |
IPTG | Stock = 0.5 M use 0.5 mM in cultures |
*NOTE: These concentrations are STOCK concentrations - not working concentrations!
Links to Labs& Project Info
Series1:
Worm Info
Lab 1: Worm Boot Camp & Sex-Linked or Autosomal Start
Lab 2: Sex-Linked or Autosomal Finale
Series2:
Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping Part 2
Lab 6: Score & DNA sequencing analysis
Series3:
Schedule of Reverse Genetics Project
RNAi General Information
Media Recipes
Lab 5: Picking your gene to RNAi
Lab 6: Cloning your gene of interest
Lab 7: Picking your transformant
Lab 8: Plasmid purification and transformation
Lab 9: Induction of bacteria for RNAi
Lab 10: Scoring your worms and RNA purification
Lab 11: RT PCR reactions