BISC 219/F10:Media Recipes: Difference between revisions

Revision as of 09:16, 15 October 2010

Wellesley College BISC 219 Genetics

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Solution	Composition
Solution 1*	50 mM glucose 10 mM EDTA (Ethylenediaminetetraacetic acid) 25 mM Tris, pH 8
Solution 2*	0.2 M NaOH 1% SDS (sodium dodecyl sulfate)
Solution 3*	2.7M Potassium Acetate 6.6M Acetic Acid
Luria-Bertoni Medium (LB)	1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar in plates
Ampicillin*	Stock = 50 mg/ml in water use 1:1000 in liquid cultures 1:500 in agar
Tetracycline*	Stock = 12.5mg/ml in 50% EtOH use 1:1000 in liquid 1:1000 in agar
IPTG	Stock = 0.5 M use 0.5 mM in cultures

*NOTE: These concentrations are STOCK concentrations - not working concentrations!

@@ Line 8: / Line 8: @@
 |-
 ! Solution 1*
-| 50 mM glucose<br> 10 mM EDTA <br> 25 mM Tris, pH 8
+| 50 mM glucose<br> 10 mM EDTA (Ethylenediaminetetraacetic acid) <br> 25 mM Tris, pH 8
 |-
 ! Solution 2*