BISC 219/F10:Media Recipes: Difference between revisions

Revision as of 14:05, 9 November 2010

Wellesley College BISC 219 Genetics

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Solution	Composition
Solution 1*	50 mM glucose 10 mM EDTA (Ethylenediaminetetraacetic acid) 25 mM Tris, pH 8
Solution 2*	0.2 M NaOH 1% SDS (sodium dodecyl sulfate)
Solution 3*	2.7M Potassium Acetate 6.6M Acetic Acid
Luria-Bertoni Medium (LB)	1% Tryptone 0.5% Yeast Extract 1% NaCl (2% agar in plates)
Ampicillin*	Stock = 50 mg/ml in water use 1:1000 in liquid cultures 1:500 in agar
Tetracycline*	Stock = 12.5mg/ml in 50% EtOH use 1:1000 in liquid 1:1000 in agar
IPTG	Stock = 0.5 M use 0.5 mM in cultures
1% agrose gel	1% (w/v) agarose in 1X TBE buffer (89mM Tris Base (TRIZMA), 089mM boric acid, 2mM EDTA (pH 8.0)
loading dye	0.4 % Ficoll 400, 1.8 mM EDTA, 0.55 mM Tris-HCl, 0.00117 % SDS, 0.025 % Bromophenol Blue pH 8.0 @ 25°C

*NOTE: These concentrations are STOCK concentrations - not working concentrations!

@@ Line 27: / Line 27: @@
 !IPTG
 | Stock = 0.5 M <br> use 0.5 mM in cultures
+|-
+! 1% agrose gel
+|1% (w/v) agarose in 1X TBE buffer (89mM Tris Base (TRIZMA), 089mM boric acid, 2mM EDTA (pH 8.0)
+|-
+! loading dye
+|0.4 % Ficoll 400, 1.8 mM EDTA, 0.55 mM Tris-HCl, 0.00117 % SDS, 0.025 % Bromophenol Blue pH 8.0 @ 25°C
 |-
 |}