BISC 219/F10:Media Recipes: Difference between revisions

Revision as of 05:18, 10 November 2010

Wellesley College BISC 219 Genetics

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Solution	Composition
Solution 1*	50 mM glucose 10 mM EDTA (Ethylenediaminetetraacetic acid) 25 mM Tris, pH 8
Solution 2*	0.2 M NaOH 1% SDS (sodium dodecyl sulfate)
Solution 3*	2.7M Potassium Acetate 6.6M Acetic Acid
Luria-Bertoni Medium (LB)	1% Tryptone 0.5% Yeast Extract 1% NaCl (2% agar in plates)
Ampicillin*	Stock = 50 mg/ml in water use 1:1000 in liquid cultures 1:500 in agar
Tetracycline*	Stock = 12.5mg/ml in 50% EtOH use 1:1000 in liquid 1:1000 in agar
IPTG	Stock = 0.5 M use 0.5 mM in cultures
1% agarose gel	1% (w/v) agarose in 1X TBE buffer (89mM Tris Base (TRIZMA), 089mM boric acid, 2mM EDTA (pH 8.0)
loading dye	0.4 % Ficoll 400, 1.8 mM EDTA, 0.55 mM Tris-HCl, 0.00117 % SDS, 0.025 % Bromophenol Blue pH 8.0 @ 25°C
1x PBS (phosphate buffered saline)	137 mM NaCl,2.7 mM KCl,4.3 mM Na2HPO4,1.47 mM KH2PO4,Adjust to a final pH of 7.4.

*NOTE: These concentrations are STOCK concentrations - not working concentrations!

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-== Plasmid Isolation and Transformation Solutions ==
+== Ingredients and Stock Concentrations for some of the reagents used in Series 3 ==
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