BISC 219/F10:Media Recipes

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Plasmid Isolation and Transformation Solutions

Solution Composition
Solution 1* 50 mM glucose
10 mM EDTA
25 mM Tris, pH 8
Solution 2* 0.2 M NaOH
1% SDS
Solution 3* 2.7M Potassium Acetate
6.6M Acetic Acid
Luria Broth 1% Tryptone
0.5% Yeast Extract
1% NaCl
2% agar in plates
Ampicillin* Stock = 50 mg/ml in water
use 1:1000 in liquid cultures
1:500 in agar
Tetracycline* Stock = 12.5mg/ml in 50% EtOH
use 1:1000 in liquid
1:1000 in agar
IPTG Stock = 0.5 M
use 0.5 mM in cultures
*NOTE: These concentrations are STOCK concentrations - not working concentrations!
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