BISC 219/F10:Media Recipes
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Plasmid Isolation and Transformation Solutions
| Solution | Composition |
|---|---|
| Solution 1* | 50 mM glucose 10 mM EDTA 25 mM Tris, pH 8 |
| Solution 2* | 0.2 M NaOH 1% SDS |
| Solution 3* | 2.7M Potassium Acetate 6.6M Acetic Acid |
| Luria Broth | 1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar in plates |
| Ampicillin* | Stock = 50 mg/ml in water use 1:1000 in liquid cultures 1:500 in agar |
| Tetracycline* | Stock = 12.5mg/ml in 50% EtOH use 1:1000 in liquid 1:1000 in agar |
| IPTG | Stock = 0.5 M use 0.5 mM in cultures |
*NOTE: These concentrations are STOCK concentrations - not working concentrations!
