BISC 219/F10:Media Recipes

Wellesley College BISC 219 Genetics

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Plasmid Isolation and Transformation Solutions


Solution	Composition
Solution 1*	50 mM glucose 10 mM EDTA 25 mM Tris, pH 8
Solution 2*	0.2 M NaOH 1% SDS
Solution 3*	2.7M Potassium Acetate 6.6M Acetic Acid
Luria Broth	1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar in plates
Ampicillin*	Stock = 50 mg/ml in water use 1:1000 in liquid cultures 1:500 in agar
Tetracycline*	Stock = 12.5mg/ml in 50% EtOH use 1:1000 in liquid 1:1000 in agar
IPTG	Stock = 0.5 M use 0.5 mM in cultures

*NOTE: These concentrations are STOCK concentrations - not working concentrations!