BISC 219/F10: Lab 2: Difference between revisions
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(New page: {{Template:BISC 219/F10}} <div style="padding: 10px; width: 720px; border: 5px solid #2171B7;"> == Lab 2: Culmination of Gene Linkage == #Examine each plate of F2 progeny. If you chose ...) |
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== Lab 2: Culmination of Gene Linkage == | == Lab 2: Culmination of Gene Linkage == | ||
Revision as of 07:59, 29 July 2010
Lab 2: Culmination of Gene Linkage
- Examine each plate of F2 progeny. If you chose only L4 hermaphrodites you should only see hermaphrodite progeny. If you have a lot of males on your plates you chose young adult worms rather than L4's - see your instructor.
- For each cross you will count and examine 100 worms - make this a random sample - the mutant worms may be smaller and not move as well as the wild types. Look around your plate.
- Record in your lab notebook the number of WT, Dpy, Unc or Dpy Unc mutants by examining the phenotype as you remove each animal from the plate (flame the pick to remove the worm). If unlinked, you should see WT's (+/+;+/+), Dpy’s (d/d;+/+), Unc’s(+/+;u/u) and Dpy Unc’s (d/d;u/u) in a ratio of 9:3:3:1. If linked, you should see a greater proportion of Dpy Unc’s (d u/d u) double mutants vs Dpy or Unc single mutants among the mutant hermaphrodite progeny.
You should now be able to conclude which strain is autosomal and linked, autosomal and unlinked and finally which strain has an x-linked gene and which one it is Dpy or Unc.
