BISC 219/F10: Lab 2 Mutant Hunt
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Mutant Hunting
To Do Today (per group)
- Choose one of the six plates at the front of the room
- Scan your “mutagenized” plate for visible dumpy mutants
- Transfer 1 putative mutant hermaphrodite to each of three new plates. Take care not to transfer any other animals except your putative mutant.
- Label these identical plates with the hermaphrodite mutant's strain information and your group's information. Use your BLUE Sharpie for labeling mapping experiment plates.
- Put an elastic around the three mapping plates. Place them in your group's plastic box (make sure your box is labeled with your names and lab section).
- Place your box on the shelf in the incubator for your lab section.
- Incubate your worms at 23°C for 3 days.
3 Days After Lab
- Examine your three plates containing your dpy worms from the mutant hunt. Are all the worms on the plates the dpy phenotype? If YES, then start a cross between 4 dpy L4 hermaphrodites (still have the clearing about 1/2 way down the body of the worm) and 3-4 wild type male worms. If NO contact your instructor ASAP!
- Set up two identical mating plates - 3-4 L4 hermaphrodites and 3-4 wild type males.
- Label these identical plates with the hermaphrodite mutant's strain information and your group's information. Use your BLUE Sharpie for labeling mapping experiment plates.
- Incubate your worms at 23°C until next lab period.