BISC 219/F10: RNAi Lab 10: Difference between revisions
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== Scoring RNAi Worms and RNA Isolation== | |||
'''3 days before next lab:''' | |||
#Come into lab and find your stack of plates. | |||
#On 2 of the experimental plates add 2 L4 wild type (N2) hermaphrodites | |||
#On 2 of the experimental plates add 2 L4 ''rrf-3'' hermaphrodites | |||
#On 1 of the control plates add 2 L4 wild type (N2) hermaphrodites | |||
#On 1 of the control plates add 2 L4 ''rrf-3'' hermaphrodites | |||
#Wrap all of your plates in an elastic and stick in your lab day box in the worm incubator set at 23°C<br> | |||
<br> | |||
== Phenotypic Analysis == | |||
Today in lab you will examine your RNAi worms and their progeny. <br> | |||
#Remove your plates from the incubator. | |||
#Examine your wild type (N2) and ''rrf-3'' RNAi worms. Do you see any difference between the two strains of worms? | |||
#Compare your RNAi worms to the control worms - are they the same phenotype? Different? | |||
#Compare your RNAi worms to worms that have a known mutation in that gene (if we have them) - how do the compare? | |||
#Score and count approximately 50 worms per RNAi plate. What is the proportion of affected to unaffected worms? | |||
<br> | |||
Be sure to record all of your results in your lab notebook.<br> | |||
Take pictures of your controla and RNAi worms for your paper.<br> | |||
<br> | |||
== RNA Isolation == |
Revision as of 19:13, 14 August 2010
Scoring RNAi Worms and RNA Isolation
3 days before next lab:
- Come into lab and find your stack of plates.
- On 2 of the experimental plates add 2 L4 wild type (N2) hermaphrodites
- On 2 of the experimental plates add 2 L4 rrf-3 hermaphrodites
- On 1 of the control plates add 2 L4 wild type (N2) hermaphrodites
- On 1 of the control plates add 2 L4 rrf-3 hermaphrodites
- Wrap all of your plates in an elastic and stick in your lab day box in the worm incubator set at 23°C
Phenotypic Analysis
Today in lab you will examine your RNAi worms and their progeny.
- Remove your plates from the incubator.
- Examine your wild type (N2) and rrf-3 RNAi worms. Do you see any difference between the two strains of worms?
- Compare your RNAi worms to the control worms - are they the same phenotype? Different?
- Compare your RNAi worms to worms that have a known mutation in that gene (if we have them) - how do the compare?
- Score and count approximately 50 worms per RNAi plate. What is the proportion of affected to unaffected worms?
Be sure to record all of your results in your lab notebook.
Take pictures of your controla and RNAi worms for your paper.