BISC 219: Mod 2 Media Recipes

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(Plasmid Isolation and Transformation Solutions)
Line 19: Line 19:
! Luria Broth
! Luria Broth
| 1% Tryptone <br> 0.5% Yeast Extract <br> 1% NaCl <br> 2% agar in plates
| 1% Tryptone <br> 0.5% Yeast Extract <br> 1% NaCl <br> 2% agar in plates
 +
|-
! Ampicillin
! Ampicillin
| Stock = 100 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
| Stock = 100 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
 +
|-
! Tetracyclin
! Tetracyclin
| Stock =    mg/ml in        <br> use 1:    in liquid  <br>  1:    in agar
| Stock =    mg/ml in        <br> use 1:    in liquid  <br>  1:    in agar
 +
|-
|}
|}

Revision as of 21:18, 26 August 2009

Wellesley College BISC 219 Genetics


Plasmid Isolation and Transformation Solutions

Solution Composition
Solution 1 50 mM glucose
10 mM EDTA
25 mM Tris, pH 8
Solution 2 0.2 M NaOH
1% SDS
Solution 3 2.7M Potassium Acetate
6.6M Acetic Acid
Luria Broth 1% Tryptone
0.5% Yeast Extract
1% NaCl
2% agar in plates
Ampicillin Stock = 100 mg/ml in water
use 1:1000 in liquid cultures
1:500 in agar
Tetracyclin Stock = mg/ml in
use 1: in liquid
1: in agar


RNAi Schedule of Experiments
RNAi General Information
Lab 4: Picking your gene to RNAi
Lab 5: Plasmid DNA isolation and transformation
Lab 6: Induction of RNAi plasmid and C. elegans feeding
Lab 7: Single Worm PCR and Agarose Gel Electrophoresis

Lab 8: PCR Reaction Cleanup and Sequencing
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