BISC 219: Mod 2 Media Recipes

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(Plasmid Isolation and Transformation Solutions)
 
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== Plasmid Isolation and Transformation Solutions ==
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{| border="1"
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! Solution !! Composition 
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|-
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! Solution 1 
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| 50 mM glucose<br> 10 mM EDTA <br> 25 mM Tris, pH 8 
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|-
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! Solution 2
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| 0.2 M NaOH <br> 1% SDS
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|-
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! Solution 3
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| 2.7M Potassium Acetate <br> 6.6M Acetic Acid
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|-
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! Luria Broth
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| 1% Tryptone <br> 0.5% Yeast Extract <br> 1% NaCl <br> 2% agar in plates
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|-
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! Ampicillin
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| Stock = 50 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
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|-
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! Tetracycline
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| Stock = 12.5mg/ml in 70% ethanol <br> N.B. tetracycline is light sensitive <br> use 1: 1000  in liquid  <br>  1:1000    in agar
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|-
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|}
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[[BISC 219/2009:RNA interference| RNAi Schedule of Experiments]]<br>
[[BISC 219/2009:RNA interference| RNAi Schedule of Experiments]]<br>

Current revision

Wellesley College BISC 219 Genetics


Plasmid Isolation and Transformation Solutions

Solution Composition
Solution 1 50 mM glucose
10 mM EDTA
25 mM Tris, pH 8
Solution 2 0.2 M NaOH
1% SDS
Solution 3 2.7M Potassium Acetate
6.6M Acetic Acid
Luria Broth 1% Tryptone
0.5% Yeast Extract
1% NaCl
2% agar in plates
Ampicillin Stock = 50 mg/ml in water
use 1:1000 in liquid cultures
1:500 in agar
Tetracycline Stock = 12.5mg/ml in 70% ethanol
N.B. tetracycline is light sensitive
use 1: 1000 in liquid
1:1000 in agar


RNAi Schedule of Experiments
RNAi General Information
Lab 4: Picking your gene to RNAi
Lab 5: Plasmid DNA isolation and transformation
Lab 6: Induction of RNAi plasmid and C. elegans feeding
Lab 7: Single Worm PCR and Agarose Gel Electrophoresis

Lab 8: PCR Reaction Cleanup and Sequencing
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