BISC 219: Mod 2 Media Recipes

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Wellesley College BISC 219 Genetics

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Gene Mapping RNA interference Creating a Transgenic Organism Assignments


Solution	Composition
Solution 1	50 mM glucose 10 mM EDTA 25 mM Tris, pH 8
Solution 2	0.2 M NaOH 1% SDS
Solution 3	2.7M Potassium Acetate 6.6M Acetic Acid
Luria Broth	1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar in plates
Ampicillin	Stock = 50 mg/ml in water use 1:1000 in liquid cultures 1:500 in agar
Tetracycline	Stock = 12.5mg/ml in 70% ethanol N.B. tetracycline is light sensitive use 1: 1000 in liquid 1:1000 in agar

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 {{Template:BISC 219/2009}}
 <div style="padding: 10px; width: 720px; border: 5px solid #2171B7;">
+== Plasmid Isolation and Transformation Solutions ==
+{| border="1"
+|+
+! Solution !! Composition
+|-
+! Solution 1
+| 50 mM glucose<br> 10 mM EDTA <br> 25 mM Tris, pH 8
+|-
+! Solution 2
+| 0.2 M NaOH <br> 1% SDS
+|-
+! Solution 3
+| 2.7M Potassium Acetate <br> 6.6M Acetic Acid
+|-
+! Luria Broth
+| 1% Tryptone <br> 0.5% Yeast Extract <br> 1% NaCl <br> 2% agar in plates
+|-
+! Ampicillin
+| Stock = 50 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
+|-
+! Tetracycline
+| Stock = 12.5mg/ml in 70% ethanol <br> N.B. tetracycline is light sensitive <br> use 1: 1000   in liquid  <br>  1:1000    in agar
+|-
+|}
 [[BISC 219/2009:RNA interference| RNAi Schedule of Experiments]]<br>