BISC 219: Mod 2 Media Recipes

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Wellesley College BISC 219 Genetics

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Gene Mapping RNA interference Creating a Transgenic Organism Assignments


Solution	Composition
Solution 1	50 mM glucose 10 mM EDTA 25 mM Tris, pH 8
Solution 2	0.2 M NaOH 1% SDS
Solution 3	2.7M Potassium Acetate 6.6M Acetic Acid
Luria Broth	1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar in plates
Ampicillin	Stock = 50 mg/ml in water use 1:1000 in liquid cultures 1:500 in agar
Tetracycline	Stock = 12.5mg/ml in 70% ethanol N.B. tetracycline is light sensitive use 1: 1000 in liquid 1:1000 in agar

@@ Line 19: / Line 19: @@
 ! Luria Broth
 | 1% Tryptone <br> 0.5% Yeast Extract <br> 1% NaCl <br> 2% agar in plates
+|-
 ! Ampicillin
-| Stock = 100 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
+| Stock = 50 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
-! Tetracyclin
+|-
-| Stock =     mg/ml in        <br> use 1:    in liquid  <br>  1:    in agar
+! Tetracycline
+| Stock = 12.5mg/ml in 70% ethanol <br> N.B. tetracycline is light sensitive <br> use 1: 1000   in liquid  <br>  1:1000    in agar
+|-
 |}