BISC 219: Mod 2 Media Recipes: Difference between revisions
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! Ampicillin | ! Ampicillin | ||
| Stock = | | Stock = 50 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar | ||
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! | ! Tetracycline | ||
| Stock = | | Stock = 12.5mg/ml in 70% ethanol <br> N.B. tetracycline is light sensitive <br> use 1: 1000 in liquid <br> 1:1000 in agar | ||
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Latest revision as of 19:34, 8 October 2012
Plasmid Isolation and Transformation Solutions
| Solution | Composition |
|---|---|
| Solution 1 | 50 mM glucose 10 mM EDTA 25 mM Tris, pH 8 |
| Solution 2 | 0.2 M NaOH 1% SDS |
| Solution 3 | 2.7M Potassium Acetate 6.6M Acetic Acid |
| Luria Broth | 1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar in plates |
| Ampicillin | Stock = 50 mg/ml in water use 1:1000 in liquid cultures 1:500 in agar |
| Tetracycline | Stock = 12.5mg/ml in 70% ethanol N.B. tetracycline is light sensitive use 1: 1000 in liquid 1:1000 in agar |
RNAi Schedule of Experiments
RNAi General Information
Lab 4: Picking your gene to RNAi
Lab 5: Plasmid DNA isolation and transformation
Lab 6: Induction of RNAi plasmid and C. elegans feeding
Lab 7: Single Worm PCR and Agarose Gel Electrophoresis
Lab 8: PCR Reaction Cleanup and Sequencing
