BISC 219: Mod 2 Media Recipes - Revision history

Dae Houlihan: /* Plasmid Isolation and Transformation Solutions */

2012-10-09T02:34:06Z

Plasmid Isolation and Transformation Solutions

←Older revision		Revision as of 02:34, 9 October 2012
Line 24:		Line 24:
	\|-		\|-
	! Tetracycline		! Tetracycline
-	\| Stock = 12.5mg/ml in 70% ethanol <br> use 1: 1000 in liquid <br> 1:1000 in agar	+	\| Stock = 12.5mg/ml in 70% ethanol <br> N.B. tetracycline is light sensitive <br> use 1: 1000 in liquid <br> 1:1000 in agar
	\|-		\|-
	\|}		\|}

Dae Houlihan: /* Plasmid Isolation and Transformation Solutions */

2012-10-09T02:32:42Z

Plasmid Isolation and Transformation Solutions

←Older revision		Revision as of 02:32, 9 October 2012
Line 24:		Line 24:
	\|-		\|-
	! Tetracycline		! Tetracycline
-	\| Stock = 12.5mg/ml in <br> use 1: 1000 in liquid <br> 1:1000 in agar	+	\| Stock = 12.5mg/ml in 70% ethanol <br> use 1: 1000 in liquid <br> 1:1000 in agar
	\|-		\|-
	\|}		\|}

Melissa Beers: /* Plasmid Isolation and Transformation Solutions */

2009-10-07T19:56:55Z

Plasmid Isolation and Transformation Solutions

←Older revision		Revision as of 19:56, 7 October 2009
Line 23:		Line 23:
	\| Stock = 50 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar		\| Stock = 50 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
	\|-		\|-
-	! ~~Tetracyclin~~	+	! Tetracycline
	\| Stock = 12.5mg/ml in <br> use 1: 1000 in liquid <br> 1:1000 in agar		\| Stock = 12.5mg/ml in <br> use 1: 1000 in liquid <br> 1:1000 in agar
	\|-		\|-

Tucker Crum: /* Plasmid Isolation and Transformation Solutions */

2009-09-29T14:18:57Z

Plasmid Isolation and Transformation Solutions

←Older revision		Revision as of 14:18, 29 September 2009
Line 24:		Line 24:
	\|-		\|-
	! Tetracyclin		! Tetracyclin
-	\| Stock = mg/ml in <br> use 1: in liquid <br> 1: in agar	+	\| Stock = 12.5mg/ml in <br> use 1: 1000 in liquid <br> 1:1000 in agar
	\|-		\|-
	\|}		\|}

Tucker Crum at 14:04, 29 September 2009

2009-09-29T14:04:22Z

←Older revision		Revision as of 14:04, 29 September 2009
Line 21:		Line 21:
	\|-		\|-
	! Ampicillin		! Ampicillin
-	\| Stock = ~~100~~ mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar	+	\| Stock = 50 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
	\|-		\|-
	! Tetracyclin		! Tetracyclin

Melissa Beers: /* Plasmid Isolation and Transformation Solutions */

2009-08-27T02:18:40Z

Plasmid Isolation and Transformation Solutions

←Older revision		Revision as of 02:18, 27 August 2009
Line 19:		Line 19:
	! Luria Broth		! Luria Broth
	\| 1% Tryptone <br> 0.5% Yeast Extract <br> 1% NaCl <br> 2% agar in plates		\| 1% Tryptone <br> 0.5% Yeast Extract <br> 1% NaCl <br> 2% agar in plates
		+	\|-
	! Ampicillin		! Ampicillin
	\| Stock = 100 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar		\| Stock = 100 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
		+	\|-
	! Tetracyclin		! Tetracyclin
	\| Stock = mg/ml in <br> use 1: in liquid <br> 1: in agar		\| Stock = mg/ml in <br> use 1: in liquid <br> 1: in agar
		+	\|-
	\|}		\|}

Melissa Beers at 02:18, 27 August 2009

2009-08-27T02:18:03Z

←Older revision		Revision as of 02:18, 27 August 2009
Line 1:		Line 1:
	{{Template:BISC 219/2009}}		{{Template:BISC 219/2009}}
	<div style="padding: 10px; width: 720px; border: 5px solid #2171B7;">		<div style="padding: 10px; width: 720px; border: 5px solid #2171B7;">
		+
		+
		+	== Plasmid Isolation and Transformation Solutions ==
		+	{\| border="1"
		+	\|+
		+	! Solution !! Composition
		+	\|-
		+	! Solution 1
		+	\| 50 mM glucose<br> 10 mM EDTA <br> 25 mM Tris, pH 8
		+	\|-
		+	! Solution 2
		+	\| 0.2 M NaOH <br> 1% SDS
		+	\|-
		+	! Solution 3
		+	\| 2.7M Potassium Acetate <br> 6.6M Acetic Acid
		+	\|-
		+	! Luria Broth
		+	\| 1% Tryptone <br> 0.5% Yeast Extract <br> 1% NaCl <br> 2% agar in plates
		+	! Ampicillin
		+	\| Stock = 100 mg/ml in water <br> use 1:1000 in liquid cultures <br> 1:500 in agar
		+	! Tetracyclin
		+	\| Stock = mg/ml in <br> use 1: in liquid <br> 1: in agar
		+	\|}
		+

	[[BISC 219/2009:RNA interference\| RNAi Schedule of Experiments]]<br>		[[BISC 219/2009:RNA interference\| RNAi Schedule of Experiments]]<br>

Melissa Beers at 01:34, 26 August 2009

2009-08-26T01:34:36Z

←Older revision		Revision as of 01:34, 26 August 2009
Line 6:		Line 6:
	[[BISC 219: Mod 2 Lab 4 \| Lab 4: Picking your gene to RNAi]]<br>		[[BISC 219: Mod 2 Lab 4 \| Lab 4: Picking your gene to RNAi]]<br>
	[[BISC 219: Mod 2 Lab 5 \| Lab 5: Plasmid DNA isolation and transformation]]<br>		[[BISC 219: Mod 2 Lab 5 \| Lab 5: Plasmid DNA isolation and transformation]]<br>
-	[[BISC 219: Mod 2 Lab 6 \| Lab 6: Induction of RNAi plasmid and ''C. elegans ~~feeding~~'']]<br>	+	[[BISC 219: Mod 2 Lab 6 \| Lab 6: Induction of RNAi plasmid and ''C. elegans'' feeding]]<br>
	[[BISC 219: Mod 2 Lab 7 \| Lab 7: Single Worm PCR and Agarose Gel Electrophoresis]]<br>		[[BISC 219: Mod 2 Lab 7 \| Lab 7: Single Worm PCR and Agarose Gel Electrophoresis]]<br>
	[[BISC 219: Mod 2 Lab 8 \| Lab 8: PCR Reaction Cleanup and Sequencing]]<br>		[[BISC 219: Mod 2 Lab 8 \| Lab 8: PCR Reaction Cleanup and Sequencing]]<br>

Melissa Beers at 01:27, 26 August 2009

2009-08-26T01:27:21Z

←Older revision		Revision as of 01:27, 26 August 2009
Line 2:		Line 2:
	<div style="padding: 10px; width: 720px; border: 5px solid #2171B7;">		<div style="padding: 10px; width: 720px; border: 5px solid #2171B7;">

-	[[~~Editing~~ BISC 219/2009:RNA interference\| RNAi Schedule of Experiments]]<br>	+	[[BISC 219/2009:RNA interference\| RNAi Schedule of Experiments]]<br>
	[[BISC 219: Mod 2 RNAi General Information\| RNAi General Information]] <br>		[[BISC 219: Mod 2 RNAi General Information\| RNAi General Information]] <br>
	[[BISC 219: Mod 2 Lab 4 \| Lab 4: Picking your gene to RNAi]]<br>		[[BISC 219: Mod 2 Lab 4 \| Lab 4: Picking your gene to RNAi]]<br>

Melissa Beers: New page: {{Template:BISC 219/2009}}
RNAi Schedule of Experiments
[[BISC 219: M...

2009-08-26T01:26:34Z

New page: {{Template:BISC 219/2009}} <div style="padding: 10px; width: 720px; border: 5px solid #2171B7;"> RNAi Schedule of Experiments<br> [[BISC 219: M...

New page

{{Template:BISC 219/2009}}
<div style="padding: 10px; width: 720px; border: 5px solid #2171B7;">

[[Editing BISC 219/2009:RNA interference| RNAi Schedule of Experiments]]<br>
[[BISC 219: Mod 2 RNAi General Information| RNAi General Information]] <br>
[[BISC 219: Mod 2 Lab 4 | Lab 4: Picking your gene to RNAi]]<br>
[[BISC 219: Mod 2 Lab 5 | Lab 5: Plasmid DNA isolation and transformation]]<br>
[[BISC 219: Mod 2 Lab 6 | Lab 6: Induction of RNAi plasmid and ''C. elegans feeding'']]<br>
[[BISC 219: Mod 2 Lab 7 | Lab 7: Single Worm PCR and Agarose Gel Electrophoresis]]<br>
[[BISC 219: Mod 2 Lab 8 | Lab 8: PCR Reaction Cleanup and Sequencing]]<br>

BISC 219: Mod 2 Media Recipes - Revision history

Dae Houlihan: /* Plasmid Isolation and Transformation Solutions */

Dae Houlihan: /* Plasmid Isolation and Transformation Solutions */

Melissa Beers: /* Plasmid Isolation and Transformation Solutions */

Tucker Crum: /* Plasmid Isolation and Transformation Solutions */

Tucker Crum at 14:04, 29 September 2009

Melissa Beers: /* Plasmid Isolation and Transformation Solutions */

Melissa Beers at 02:18, 27 August 2009

Melissa Beers at 01:34, 26 August 2009

Melissa Beers at 01:27, 26 August 2009

Melissa Beers: New page: {{Template:BISC 219/2009}} RNAi Schedule of Experiments [[BISC 219: M...

Melissa Beers: New page: {{Template:BISC 219/2009}}
RNAi Schedule of Experiments
[[BISC 219: M...