BMCB625:DNA Gyrase
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Discussion/Homework Questions
Mahta
Larry
Jon
Jeremy
Chris
Discussion point: Historically, DNA circles have been found to be underwound, that is “their linking numbers are less than those of their corresponding relaxed circles. This phenomena has been established by observing the effect of ethidium ion (from EtBr) affecting the rate of sedimentation (Voet & Voet 3/e).” This has been shown experimentally by plotting the Buoyant density as a function of EtBr concentration (in ug/uL). How does today’s paper support this biophysical finding? File:Http://www.web.virginia.edu/Heidi/chapter12/Images/8883n12 16.jpg