BMCB625:DNA Replication
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(Homework) Questions
Larry
Lopes et al
- What observations lead to the authors' suggestion that discontinuites left randomly behind the fork are not immediately sealed after the passage of the replication fork? Larry's Question Page
Heller and Marians
- How did the author cleverly show that the same DnaB from the lagging strand was involved in leading strand reinitation? Larry's Question Page
Chayne
Jon
Chris
Lopes et al (UV-irrad of S. cerevisiae; EM grids and 2-D gel electrophoresis)
- Lopes and colleagues never actually demonstrate that the ssDNA is located on the strand opposite the UV photoproducts. Suggest a possible way in which this may be elucidated.
Heller and Marians (Biochemical purification and modifications from E.coli and chemical Mods.)
- Topic for Discussion Thursday: It appears in both papers that specialized translesion polymerases are needed. How broadly applicable are these proposed mechanisms (i.e., can we really assume that what occurs in a severely damaged DNA strand is the same process as “healthy” DNA synthesis? Are they specific to single-celled organisms which do not participate in the complex process of apoptosis that is found in multi-cellular organisms)?
--Chris 00:55, 9 April 2007 (EDT)
Mahta
Jeremy
Lopes et al
Despite the remarkable discovery that DNA polymerase can transcribe through UV lesions when yeast cells lack NER, list potential genotoxic consequences of transcribing through these lesions?
Heller and Marians
Why might the mechanism described by Heller and Marians be considered heretical?
